Document Detail


Cardiac myocyte nuclear size and ploidy status decrease after mechanical support.
MedLine Citation:
PMID:  11425598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two patients with end-stage dilated cardiomyopathy of ischemic and idiopathic origin were treated with a left ventricular assist device (LVAD) as a bridge for heart transplantation. Myocardial tissue was collected during LVAD insertion and from the left ventricular apex of the explanted hearts. The myocyte diameter, nuclear area and DNA content of myocyte nuclei were measured by static cytomorphometry in tissue sections and in isolated myocytes with a digital analysis system. The presence of apoptotic nuclei was investigated by the TdT mediated X-dUTP nick end labeling technique (TUNEL). The prolonged use of a LVAD was associated with a reduction in myocyte diameter, indicating that the LVAD may induce a reversion of myocyte hypertrophy, a process described as "reverse remodeling." In addition, unloading of the heart induced a reduction in the size and DNA content of myocyte nuclei. These results suggest that the cardiomyocyte nuclei are in a dynamic state and, as it occurs with cell hypertrophy, nuclear hypertrophy and polyploidization may be a reversible phenomenon.
Authors:
H G Rivello; P C Meckert; C Vigliano; R Favaloro; R P Laguens
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology     Volume:  10     ISSN:  1054-8807     ISO Abbreviation:  Cardiovasc. Pathol.     Publication Date:    2001 Mar-Apr
Date Detail:
Created Date:  2001-06-26     Completed Date:  2001-08-09     Revised Date:  2006-11-03    
Medline Journal Info:
Nlm Unique ID:  9212060     Medline TA:  Cardiovasc Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-7     Citation Subset:  IM    
Affiliation:
Cátedra de Patología, Universidad Nacional de La Plata, Argentina. hgarciarivello@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Apoptosis
Cardiomyopathy, Dilated / genetics,  therapy*
Cell Nucleus / pathology*
DNA / analysis
Heart-Assist Devices*
Humans
Image Cytometry
Image Processing, Computer-Assisted
In Situ Nick-End Labeling
Male
Middle Aged
Myocardium / pathology*
Ploidies*
Ventricular Remodeling / physiology
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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