Document Detail

Cardiac microdialysis in isolated rat hearts: interstitial purine metabolites during ischemia.
MedLine Citation:
PMID:  1621849     Owner:  NLM     Status:  MEDLINE    
Cardiac microdialysis is a recently developed technique that allows intramyocardial interstitial fluid (ISF) to be sampled via the implantation and perfusion of a small, hollow dialysis fiber within the myocardium. The purpose of this paper is to describe initial studies using cardiac microdialysis in the isolated perfused heart. Microdialysis probes, constructed in the laboratory, were implanted in the left ventricular myocardium of isolated perfused rat hearts and perfused at 0.5 microliter/min with Krebs-Henseleit buffer. The effluent dialysate, assayed for adenosine, inosine, hypoxanthine, xanthine, and uric acid, was used as an index of intramyocardial levels of these purine metabolites. All metabolites were elevated initially after implantation, declined rapidly in the first 45 min, and were then stable for the next 90 min. Based on in vitro percent recovery data, baseline dialysate concentrations were extrapolated to yield estimates of intramyocardial ISF (in microM) 0.47 adenosine, 0.85 inosine, 0.29 hypoxanthine, 0.49 xanthine, and 8.6 uric acid. During global zero-flow ischemia (37 degrees C), dialysate levels of all purine metabolites were elevated, with inosine being the predominant compound. Pretreatment of the hearts with 50 microM erythro-9-(2-hydroxy-3-nonyl)adenine, an adenosine deaminase inhibitor, markedly enhanced ISF adenosine accumulation and attenuated the accumulation of inosine, hypoxanthine, and xanthine. The simplicity and versatility of cardiac microdialysis in the isolated perfused heart suggest that this technique may be a valuable adjunct to the many studies performed using this preparation.
D G Van Wylen; T J Schmit; R D Lasley; R L Gingell; R M Mentzer
Related Documents :
7967679 - Myocardial buffering capacity and high-energy phosphate utilization during hypothermic ...
2912189 - Relation between phosphate metabolites and oxygen consumption of heart in vivo.
3383379 - Formation of s-adenosylhomocysteine in the heart. ii: a sensitive index for regional my...
2453739 - Inotropic interactions of dichloroacetate with amrinone and ouabain in isolated hearts ...
2733029 - Total ischemia iii: effect of inhibition of anaerobic glycolysis.
3990319 - The role of cardioplegic solution buffering in myocardial protection. a biochemical and...
1523029 - Temperature affects mechanics and stability during initial inflation-deflation of matur...
2208799 - Influence of cardiac afferents on time-dependent changes in the renal sympathetic baror...
1863049 - Ventilation with isoflurane in the triservice anaesthetic apparatus: a comparison with ...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  262     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-08-04     Completed Date:  1992-08-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1934-8     Citation Subset:  IM    
Department of Physiology, School of Medicine, State University of New York, Buffalo.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenine / analogs & derivatives,  pharmacology
Coronary Disease / metabolism*
Dialysis / methods
Extracellular Space / metabolism*
Myocardium / metabolism*
Osmolar Concentration
Purines / metabolism*
Grant Support
Reg. No./Substance:
0/Purines; 59262-86-1/9-(2-hydroxy-3-nonyl)adenine; 73-24-5/Adenine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mathematical modeling of human cardiovascular system for simulation of orthostatic response.
Next Document:  Simple circuit for pacing hearts of experimental animals.