| Cardiac and metabolic changes in long-term high fructose-fat fed rats with severe obesity and extensive intramyocardial lipid accumulation. | |
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MedLine Citation:
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PMID: 20357025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Metabolic syndrome and obesity-related diseases are affecting more and more people in the Western world. The basis for an effective treatment of these patients is a better understanding of the underlying pathophysiology. Here, we characterize fructose- and fat-fed rats (FFFRs) as a new animal model of metabolic syndrome. Sprague-Dawley rats were fed a 60 kcal/100 kcal fat diet with 10% fructose in the drinking water. After 6, 12, 18, 24, 36, and 48 wk of feeding, blood pressure, glucose tolerance, plasma insulin, glucose, and lipid levels were measured. Cardiac function was examined by in vivo pressure volume measurements, and intramyocardial lipid accumulation was analyzed by confocal microscopy. Cardiac AMP-activated kinase (AMPK) and hepatic phosphoenolpyruvate carboxykinase (PEPCK) levels were measured by Western blotting. Finally, an ischemia-reperfusion study was performed after 56 wk of feeding. FFFRs developed severe obesity, decreased glucose tolerance, increased serum insulin and triglyceride levels, and an initial increased fasting glucose, which returned to control levels after 24 wk of feeding. The diet had no effect on blood pressure but decreased hepatic PEPCK levels. FFFRs showed significant intramyocardial lipid accumulation, and cardiac hypertrophy became pronounced between 24 and 36 wk of feeding. FFFRs showed no signs of cardiac dysfunction during unstressed conditions, but their hearts were much more vulnerable to ischemia-reperfusion and had a decreased level of phosphorylated AMPK at 6 wk of feeding. This study characterizes a new animal model of the metabolic syndrome that could be beneficial in future studies of metabolic syndrome and cardiac complications. |
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Authors:
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Lene N Axelsen; Jacob B Lademann; J?rgen S Petersen; Niels-Henrik Holstein-Rathlou; Thorkil Ploug; Clara Prats; Henrik D Pedersen; Anne Louise Kj?lbye |
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Publication Detail:
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Type: Journal Article Date: 2010-03-31 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 298 ISSN: 1522-1490 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-25 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1560-70 Citation Subset: IM |
Affiliation:
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Zealand Pharma A/S, Glostrup, Denmark. lax@mfi.ku.dk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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AMP-Activated Protein Kinases
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metabolism Animals Blood Pressure / drug effects Fats / pharmacology* Fructose / pharmacology* Glucose / metabolism Heart / drug effects Insulin / blood Lipids / blood Liver / metabolism Male Metabolic Syndrome X / metabolism Myocardium / metabolism Obesity / metabolism* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Fats; 0/Lipids; 11061-68-0/Insulin; 30237-26-4/Fructose; 50-99-7/Glucose; EC 2.7.11.1/AMP-Activated Protein Kinases |
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