Document Detail


Cardiac Applications of Optogenetics.
MedLine Citation:
PMID:  25035999     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In complex multicellular systems, such as the brain or the heart, the ability to selectively perturb and observe the response of individual components at the cellular level and with millisecond resolution in time, is essential for mechanistic understanding of function. Optogenetics uses genetic encoding of light sensitivity (by the expression of microbial opsins) to provide such capabilities for manipulation, recording, and control by light with cell specificity and high spatiotemporal resolution. As an optical approach, it is inherently scalable for remote and parallel interrogation of biological function at the tissue level; with implantable miniaturized devices, the technique is uniquely suitable for in vivo tracking of function, as illustrated by numerous applications in the brain. Its expansion into the cardiac area has been slow. Here, using examples from published research and original data, we focus on optogenetics applications to cardiac electrophysiology, specifically dealing with the ability to manipulate membrane voltage by light with implications for cardiac pacing, cardioversion, cell communication, and arrhythmia research, in general. We discuss gene and cell delivery methods of inscribing light sensitivity in cardiac tissue, functionality of the light-sensitive ion channels within different types of cardiac cells, utility in probing electrical coupling between different cell types, approaches and design solutions to all-optical electrophysiology by the combination of optogenetic sensors and actuators, and specific challenges in moving towards in vivo cardiac optogenetics.
Authors:
Christina M Ambrosi; Aleksandra Klimas; Jinzhu Yu; Emilia Entcheva
Related Documents :
24433169 - Mutants of phospholipase a (ppla-i) have a red light and auxin phenotype.
23015209 - Correlation properties of light backscattered multiply from fractal aggregates of parti...
21363939 - Androgens modulate structure and function of the suprachiasmatic nucleus brain clock.
25087009 - Dealing with light: the widespread and multitasking cryptochrome/photolyase family in p...
7942659 - Angle class ii, division 1 malocclusion.
23145279 - The role of head movements and signal spectrum in an auditory front/back illusion.
19872169 - The inhibition of cypridina luminescence by light.
3083489 - Frequency dependence of dead space during high-frequency ventilation in dogs.
21218889 - Recovery of distortion-product otoacoustic emissions after a 2-khz monaural sound-expos...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-15
Journal Detail:
Title:  Progress in biophysics and molecular biology     Volume:  -     ISSN:  1873-1732     ISO Abbreviation:  Prog. Biophys. Mol. Biol.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401233     Medline TA:  Prog Biophys Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier Ltd.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolation of diploid baker's yeast capable of strongly activating immune cells and analyses of the c...
Next Document:  A novel dinuclear ruthenium(ii)-copper(ii) complex-based luminescent probe for hydrogen sulfide.