Document Detail

Carcinogenic potency of perfluorooctane sulfonate (PFOS) on Syrian hamster embryo (SHE) cells.
MedLine Citation:
PMID:  22057587     Owner:  NLM     Status:  Publisher    
Perfluorooctane sulfonate (PFOS) is the degradation product of many fluoroderivatives and a widespread environmental contaminant. Its persistence, its long half-life in humans and its toxicity explain high concerns on human health side effects in future. PFOS is suspected to be a non-genotoxic carcinogen. In the present work, we assessed carcinogenic potential of PFOS by studying morphological transformation in Syrian hamster embryo (SHE) cells; cell transformation of SHE cells is an in vitro assay recommended by the Organization for Economic Cooperation and Development to detect carcinogens, genotoxic or not. Genotoxicity of PFOS and expression of PPARs genes in SHE cells were also measured. PFOS was shown to induce cell transformation (P < 0.05) at non-cytotoxic concentrations (0.2 and 2 μg/mL) (P ≤ 0.01). No genotoxic effect was recorded in the range of PFOS concentrations tested (2 × 10(-4) to 50 μg/mL) using the single-cell gel electrophoresis (comet) assay after 5 and 24 h of exposure. The expression of PPARs genes was measured by qPCR within the first 24 h and after 7 days of PFOS treatment. Results indicated an increased expression of ppar-β/δ isoform as early as 24 h. After 7 days, the increase of ppar-β/δ mRNA was significant at the concentrations inducing cell transformation (0.2 and 2 μg/mL), while overexpression of ppar-γ and ppar-α did not closely relate to effective concentrations. The results indicate that PFOS behave as a non-genotoxic carcinogen and impacted PPARs genes. Its cell transforming potential paralleled an increased expression of ppar-β/δ.
N Jacquet; M A Maire; Y Landkocz; P Vasseur
Related Documents :
19447207 - Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal tra...
11588157 - Galactosylation of n-linked oligosaccharides by human beta-1,4-galactosyltransferases i...
8874827 - Effect of transfection with a superoxide dismutase expression plasmid on xanthine/xanth...
15111587 - Effect of overexpressing the transcription factor e2f2 on cell cycle progression in rab...
47817 - Cellular proliferation and renewal in the various zones of the hamster epididymis after...
24649077 - The s protein of hepatitis b virus promotes collagen type i expression in hepatic stell...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-6
Journal Detail:
Title:  Archives of toxicology     Volume:  -     ISSN:  1432-0738     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417615     Medline TA:  Arch Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
CNRS UMR 7146, Laboratory Interactions Ecotoxicology Biodiversity Ecosystems (LIEBE), University of Metz, Rue du General Delestraint, 57070, Metz, France,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Synthesis of 3-substituted 1,5-aldehyde esters via an organocatalytic highly enantioselective conjug...
Next Document:  Constraints for monocyte derived dendritic cell functions under inflammatory conditions.