Document Detail


Carboxylic acids as biomarkers of Biomphalaria alexandrina snails infected with Schistosoma mansoni.
MedLine Citation:
PMID:  20585528     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biomphalaria alexandrina snails play an indispensable role in transmission of schistosomiasis. Infection rates in field populations of snails are routinely determined by cercarial shedding neglecting prepatent snail infections, because of lack of a suitable method for diagnosis. The present study aimed at separation and quantification of oxalic, malic, acetic, pyruvic, and fumaric acids using ion-suppression reversed-phase high performance liquid chromatography (HPLC) to test the potentiality of these acids to be used as diagnostic and therapeutic biomarkers. The assay was done in both hemolymph and digestive gland-gonad complex (DGG) samples in a total of 300 B. alexandrina snails. All of the studied acids in both the hemolymph and tissue samples except for the fumaric acid in hemolymph appeared to be good diagnostic biomarkers as they provide not only a good discrimination between the infected snails from the control but also between the studied stages of infection from each other. The most sensitive discriminating acid was malic acid in hemolymph samples as it showed the highest F-ratio. Using the Z-score, malic acid was found to be a good potential therapeutic biomarker in the prepatency stage, oxalic acid and acetic acid in the stage of patency, and malic acid and acetic acid at 2 weeks after patency. Quantification of carboxylic acids, using HPLC strategy, was fast, easy, and accurate in prediction of infected and uninfected snails and possibly to detect the stage of infection. It seems also useful for detection of the most suitable acids to be used as drug targets.
Authors:
Salwa M F Abou Elseoud; Nashwa S Abdel Fattah; Hayam M Ezz El Din; Hala Abdel Al; Hanan Mossalem; Noha Elleboudy
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Publication Detail:
Type:  Journal Article     Date:  2010-06-17
Journal Detail:
Title:  The Korean journal of parasitology     Volume:  48     ISSN:  1738-0006     ISO Abbreviation:  Korean J. Parasitol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-09-23     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9435800     Medline TA:  Korean J Parasitol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  127-32     Citation Subset:  IM    
Affiliation:
Parasitology Department, Ain Shams University, Cairo, Egypt.
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MeSH Terms
Descriptor/Qualifier:
Animal Structures / chemistry*
Animals
Biological Markers
Biomphalaria / parasitology*
Carboxylic Acids / analysis*
Chromatography, Liquid / methods
Hemolymph / chemistry*
Schistosoma mansoni / growth & development*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Carboxylic Acids
Comments/Corrections

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