| Carboxylated magnetic microbead-assisted fluoroimmunoassay for early biomarkers of acute myocardial infarction. | |
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MedLine Citation:
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PMID: 19398193 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A carboxylated superparamagnetic microbead-assisted sandwich fluoroimmunoassay was successfully demonstrated for the analysis of the early protein markers, myoglobin and human heart-type fatty acid-binding protein (H-FABP), associated with acute myocardial infarction. This assay approach consisted of the preparation of superparamagnetic polymer microbeads using a dispersion polymerization, followed by grafting of capture antibodies (monoclonal anti-H-FABP 10E1 and anti-myoglobin 7C3) onto the polymer microbeads using EDC-NHS protocol, and then a sequential sandwich fluoroimmunoassay using detection antibodies (FITC-labeled anti-H-FABP 9F3 and FITC-labeled anti-myoglobin 4E2). The Fe(3)O(4) nanoparticles and carboxylated Fe(3)O(4)-polymer microbeads were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrophotometry, vibrating sample magnetometry, and X-ray diffraction. The fluoroimmunoassay images were recorded using a confocal laser-scanning microscope, and the average fluorescence intensity of the microbeads was found to correspond to the concentration of each cardiac marker, in agreement with the results obtained by a spectrofluorophotometer. The carboxylated magnetic microbead-assisted protocol could be utilized to semi-quantitatively detect both myoglobin and H-FABP. |
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Authors:
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Jinyi Wang; Qiang Wang; Li Ren; Xueqin Wang; Zongfang Wan; Wenming Liu; Li Li; Huiying Zhao; Manlin Li; Dewen Tong; Juan Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-02 |
Journal Detail:
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Title: Colloids and surfaces. B, Biointerfaces Volume: 72 ISSN: 1873-4367 ISO Abbreviation: Colloids Surf B Biointerfaces Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-06-03 Completed Date: 2009-07-29 Revised Date: 2009-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9315133 Medline TA: Colloids Surf B Biointerfaces Country: Netherlands |
Other Details:
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Languages: eng Pagination: 112-20 Citation Subset: IM |
Affiliation:
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College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China. jywang@nwsuaf.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylates
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chemistry Acute Disease Antibodies Biological Markers / analysis Carboxylic Acids / metabolism* Ferric Compounds / metabolism Fluorescence Humans Immunoassay / methods* Magnetics* Microspheres* Myocardial Infarction / diagnosis* Nanoparticles / ultrastructure Polystyrenes / chemistry Spectroscopy, Fourier Transform Infrared Time Factors X-Ray Diffraction |
| Chemical | |
Reg. No./Substance:
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0/Acrylates; 0/Antibodies; 0/Biological Markers; 0/Carboxylic Acids; 0/Ferric Compounds; 0/Polystyrenes; 1309-37-1/ferric oxide; 79-10-7/acrylic acid |
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