Document Detail


Carbons from choline present in the phospholipids of Pseudomonas aeruginosa.
MedLine Citation:
PMID:  9513276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phospholipid composition of Pseudomonas aeruginosa grown in a mineral medium with choline as the carbon source was: phosphatidylethanolamine, 71.6 +/- 1.4%; phosphatidylglycerol, 11.8 +/- 0.4%; diphosphatidylglycerol, 0.8 +/- 0.4%; phosphatidic acid, 2.4 +/- 0.6%; lysophosphatidylethanolamine, 1.6 +/- 0.3%; phosphatidylcholine 7.9 +/- 0.3%; lysophosphatidylcholine, 3.9 +/- 0.7%. The molar ratio between the acidic and the neutral phospholipids was 0.18. Radiolabeling experiments with [methyl-14C]choline or [1,2-14C]choline carried out in cell suspension from bacteria that were grown in the presence of choline as the sole carbon source demonstrated that the carbons of the N-methyl groups of choline contributed to the synthesis of fatty acids while the carbons comprising the backbone of choline were used for the synthesis of glycerol.
Authors:
S T Albelo; C E Domenech
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  156     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-04-13     Completed Date:  1998-04-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  271-4     Citation Subset:  IM    
Affiliation:
Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Carbon / metabolism*
Carbon Radioisotopes / diagnostic use
Choline / metabolism*
Phospholipids / analysis*,  metabolism
Pseudomonas aeruginosa / chemistry*,  metabolism*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Phospholipids; 62-49-7/Choline; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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