Document Detail


Carbonic anhydrase-related protein VIII promotes colon cancer cell growth.
MedLine Citation:
PMID:  17219437     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increased expression of carbonic anhydrase-related protein (CA-RP) VIII has previously been shown in colorectal carcinoma. Since CA-RP has no catalytic carbonic anhydrase (CA) activity, the present study attempted to elucidate its biological significance in colon cancer cells. From a colon cancer cell line (LoVo), we established clones that overexpressed CA-RP VIII (LoVo-CA8) and a control transfectant with a vector alone (LoVo-pCIneo) and studied alterations in the biological behaviors of the tumor cells both in vitro and in vivo. LoVo-CA8 cells showed significantly increased mRNA and protein expressions of CA-RP VIII as compared to LoVo-pCIneo cells. Cell proliferation, colony formation, and cell invasion assays showed that LoVo-CA8 cells had significantly higher cell proliferative and invasive abilities as compared to parental LoVo and LoVo-pCIneo cells in vitro. In an in vivo xenograft assay, LoVo-CA8 cells showed a higher tumor growth rate than parental LoVo cells. Further, small interfering RNA (siRNA)-mediated knockdown of CA-RP VIII revealed significant inhibition in cell proliferation and colony formation of a colon cancer cell line HCT116, which showed high endogenous expression of CA-RP VIII. These findings indicated that CA-RP VIII plays a role in the growth of colon cancer cells.
Authors:
Makoto Nishikata; Isao Nishimori; Keisuke Taniuchi; Tamotsu Takeuchi; Tomoko Minakuchi; Takuhiro Kohsaki; Yoshihiro Adachi; Yuji Ohtsuki; Saburo Onishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular carcinogenesis     Volume:  46     ISSN:  0899-1987     ISO Abbreviation:  Mol. Carcinog.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-28     Completed Date:  2007-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8811105     Medline TA:  Mol Carcinog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  208-14     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley-Liss, Inc.
Affiliation:
Department of Gastroenterology and Hepatology, Kochi Medical School, Nankoku, Kochi, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Cell Proliferation
Colonic Neoplasms / genetics,  metabolism,  pathology*
Colony-Forming Units Assay
Gene Expression Regulation, Neoplastic
Gene Silencing / drug effects
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Invasiveness / pathology
Nerve Tissue Proteins / physiology*
RNA, Messenger / metabolism
RNA, Neoplasm / genetics
RNA, Small Interfering / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Markers, Biological / physiology*
Chemical
Reg. No./Substance:
0/CA8 protein, human; 0/Car8 protein, mouse; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/RNA, Small Interfering; 0/Tumor Markers, Biological

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