Document Detail

Carbonic anhydrase-related protein VIII increases invasiveness of non-small cell lung adenocarcinoma.
MedLine Citation:
PMID:  16609906     Owner:  NLM     Status:  MEDLINE    
Carbonic anhydrase-related protein VIII (CA-RP VIII) is believed to be an oncofetal antigen and is overexpressed in colorectal and non-small cell lung cancer. However, the pathobiological properties of CA-RP VIII in lung cancer remain unclear. In the present study, we examined ultrastructural changes caused by exogenous CA-RP VIII expression in a well-differentiated lung adenocarcinoma cell line, PC-9. Many vacuoles lined by cilia, sometimes large vacuoles pushing the nuclei to one side, were found in the cytoplasm of CA-RP VIII-expressing PC-9 cells, but not in control PC-9 cells. Moreover, signet-ring cells containing abundant intracytoplasmic mucin were often found among CA-RP VIII-expressing PC-9 cells, but rarely among control PC-9 cells. We subsequently examined CA-RP VIII expression in atypical adenomatous hyperplasia and early-stage lung adenocarcinoma (Stage Ia). Significant expression of CA-RP VIII was observed in invasive lung adenocarcinoma but not in noninvasive adenocarcinoma. Interestingly, CA-RP VIII was strongly expressed in signet-ring cell cancer and invasive mucinous adenocarcinoma components. CA-RP VIII also appeared to enhance the invasiveness of PC-9 cells in Matrigel invasion assay. The present findings suggest that CA-RP VIII expression in lung adenocarcinoma is related to cancer cell invasion.
Tsuyoshi Ishihara; Tamotsu Takeuchi; Isao Nishimori; Yoshihiro Adachi; Tomoko Minakuchi; Jiro Fujita; Hiroshi Sonobe; Yuji Ohtsuki; Saburo Onishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-12
Journal Detail:
Title:  Virchows Archiv : an international journal of pathology     Volume:  448     ISSN:  0945-6317     ISO Abbreviation:  Virchows Arch.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-31     Completed Date:  2006-11-02     Revised Date:  2006-11-20    
Medline Journal Info:
Nlm Unique ID:  9423843     Medline TA:  Virchows Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  830-7     Citation Subset:  IM    
Department of Gastroenterology and Hepatology, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
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MeSH Terms
Adenoma / genetics,  ultrastructure
Carbonic Anhydrases / genetics*
Carcinoma, Non-Small-Cell Lung / genetics,  ultrastructure*
Cell Line, Tumor
Cell Movement
Gene Expression
Gene Expression Regulation, Neoplastic*
Lung Neoplasms / genetics,  ultrastructure*
Microscopy, Electron, Transmission
Mucins / metabolism
Neoplasm Invasiveness
Nerve Tissue Proteins / genetics*
RNA, Messenger / metabolism
RNA, Neoplasm / genetics
Reverse Transcriptase Polymerase Chain Reaction
Tumor Markers, Biological
Vacuoles / ultrastructure
Reg. No./Substance:
0/CA8 protein, human; 0/Car8 protein, mouse; 0/Mucins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Tumor Markers, Biological; EC Anhydrases

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