Document Detail


Carbon storage regulator A (CsrA(Bb)) is a repressor of Borrelia burgdorferi flagellin protein FlaB.
MedLine Citation:
PMID:  21999436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Lyme disease spirochete Borrelia burgdorferi lacks the transcriptional cascade control of flagellar protein synthesis common to other bacteria. Instead, it relies on a post-transcriptional mechanism to control its flagellar synthesis. The underlying mechanism of this control remains elusive. A recent study reported that the increased level of BB0184 (CsrA(Bb); a homologue of carbon storage regulator A) substantially inhibited the accumulation of FlaB, the major flagellin protein of B. burgdorferi. In this report, we deciphered the regulatory role of CsrA(Bb) on FlaB synthesis and the mechanism involved by analysing two mutants, csrA(Bb)(-) (a deletion mutant of csrA(Bb)) and csrA(Bb)(+) (a mutant conditionally overexpressing csrA(Bb)). We found that FlaB accumulation was significantly inhibited in csrA(Bb)(+) but was substantially increased in csrA(Bb)(-) . In contrast, the levels of other flagellar proteins remained unchanged. Cryo-electron tomography and immuno-fluorescence microscopic analyses revealed that the altered synthesis of CsrA(Bb) in these two mutants specifically affected flagellar filament length. The leader sequence of flaB transcript contains two conserved CsrA-binding sites, with one of these sites overlapping the Shine-Dalgarno sequence. We found that CsrA(Bb) bound to the flaB transcripts via these two binding sites, and this binding inhibited the synthesis of FlaB at the translational level. Taken together, our results indicate that CsrA(Bb) specifically regulates the periplasmic flagellar synthesis by inhibiting translation initiation of the flaB transcript.
Authors:
Ching Wooen Sze; Dustin R Morado; Jun Liu; Nyles W Charon; Hongbin Xu; Chunhao Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2011-10-18
Journal Detail:
Title:  Molecular microbiology     Volume:  82     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-09     Completed Date:  2012-02-22     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  851-64     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
5' Untranslated Regions
Binding Sites
Borrelia burgdorferi / genetics,  metabolism,  physiology*
Cryoelectron Microscopy
Electron Microscope Tomography
Flagella / ultrastructure
Flagellin / biosynthesis*
Gene Deletion
Gene Expression
Gene Expression Regulation, Bacterial*
Microscopy, Fluorescence
Mutation
Repressor Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
AI073354/AI/NIAID NIH HHS; AI078958/AI/NIAID NIH HHS; AI087946/AI/NIAID NIH HHS; AI29743/AI/NIAID NIH HHS; R01 AI078958/AI/NIAID NIH HHS; R01 AI078958-01/AI/NIAID NIH HHS; R01 AI078958-02/AI/NIAID NIH HHS; R01 AI078958-02S1/AI/NIAID NIH HHS; R01 AI078958-03/AI/NIAID NIH HHS; R01 AI078958-04/AI/NIAID NIH HHS; R01 AI087946/AI/NIAID NIH HHS; R01 DE019667/DE/NIDCR NIH HHS; R01 DE019667-01A1/DE/NIDCR NIH HHS; R01 GM072004/GM/NIGMS NIH HHS; R21 AI073354/AI/NIAID NIH HHS; R21 AI073354-01A1/AI/NIAID NIH HHS; R21 AI073354-02/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/5' Untranslated Regions; 0/Repressor Proteins; 12777-81-0/Flagellin; 140470-87-7/flaB flagellin
Comments/Corrections

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