Document Detail

Carbon and nitrogen isotope analysis of atrazine and desethylatrazine at sub-microgram per liter concentrations in groundwater.
MedLine Citation:
PMID:  23274558     Owner:  NLM     Status:  Publisher    
Environmental degradation of organic micropollutants is difficult to monitor due to their diffuse and ubiquitous input. Current approaches-concentration measurements over time, or daughter-to-parent compound ratios-may fall short, because they do not consider dilution, compound-specific sorption characteristics or alternative degradation pathways. Compound-specific isotope analysis (CSIA) offers an alternative approach based on evidence from isotope values. Until now, however, the relatively high limits for precise isotope analysis by gas chromatography-isotope ratio mass spectrometry (GC-IRMS) have impeded CSIA of sub-microgram-per-liter scale micropollutant concentrations in field samples. This study presents the first measurements of C and N isotope ratios of the herbicide atrazine and its metabolite desethylatrazine at concentrations of 100 to 1,000 ng/L in natural groundwater samples. Solid-phase extraction and preparative HPLC were tested and validated for preconcentration and cleanup of groundwater samples of up to 10 L without bias by isotope effects. Matrix interferences after solid-phase extraction could be greatly reduced by a preparative HPLC cleanup step prior to GC-IRMS analysis. Sensitivity was increased by a factor of 6 to 8 by changing the injection method from large-volume to cold-on-column injection on the GC-IRMS system. Carbon and nitrogen isotope values of field samples showed no obvious correlation with concentrations or desethylatrazine-to-atrazine ratios. Contrary to expectations, however, δ (13) C values of desethylatrazine were consistently less negative than those of atrazine from the same sites. Potentially, this line of evidence may contain information about further desethylatrazine degradation. In such a case, the common practice of using desethylatrazine-to-atrazine ratios would underestimate natural atrazine degradation.
Kathrin Schreglmann; Martina Hoeche; Sibylle Steinbeiss; Sandra Reinnicke; Martin Elsner
Related Documents :
18614358 - Flow injection analysis-based methodology for automatic on-line monitoring and quality ...
24269478 - Asymmetric synthesis and effect of absolute stereochemistry of ycz-2013, a brassinoster...
2846518 - Presence of phosphatidylcholine hydroperoxide in human plasma.
17495408 - Flow injection chemiluminescence determination of imipramine in tablets, human plasma a...
20550108 - Real-time gas-phase analysis of mono- to tri-chlorobenzenes generated from heated mswi ...
23635828 - Effect of hbe heterozygosity on the measurement of hba1c.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-30
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  -     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Groundwater Ecology, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Graphene oxide nanoribbons (GNO), reduced graphene nanoribbons (GNR), and multi-layers of oxidized g...
Next Document:  Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modifie...