| Carbon monoxide-induced early thrombolysis contributes to heme oxygenase-1-mediated inhibition of neointimal growth after vascular injury in hypercholesterolemic mice. | |
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MedLine Citation:
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PMID: 16783602 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Arterial thrombosis is a critical event in the pathogenesis of lesion development. In this study, we evaluated the effect of heme oxygenase-1 (HO-1), a stress-inducible enzyme with vasoprotective functions, on arterial thrombosis following vascular mechanical injury. The carotid arteries of apoE-deficient mice were subjected to angioplasty with a modified beaded-needle. Arterial thrombosis occurred at 12 h after injury. Treatment of the injured vessels with an adenovirus bearing HO-1 gene (Adv-HO-1) (1 x 10(8) pfu), but not saline or empty adenovirus (Adv), immediately after angioplasty resulted in earlier thrombolysis and restoration of blood flow detected at 24 h. Immunohistochemistry revealed that the arterial plasminogen activator inhibitor-1 (PAI-1) expression was markedly reduced in Adv-HO-1-treated injured arteries as compared to control counterparts. The thrombolytic effect was also observed by exposing animals with existing arterial thrombosis to carbon monoxide (CO) (250 ppm, 2 h), a byproduct derived from heme degradation by HO-1. In parallel with less fibrin(ogen) deposition, the macrophage infiltration, monocyte chemoattractant protein-1 expression and neointimal formation assessed at 2 weeks after angioplasty were substantially reduced in injured arteries treated with Adv-HO-1. These results support a role of early thrombolysis induced by CO in HO-1-mediated protection against intimal hyperplasia after vascular injury. |
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Authors:
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Yen-Hui Chen; Hui-Ling Tsai; Ming-Tsai Chiang; Lee-Young Chau |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-17 |
Journal Detail:
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Title: Journal of biomedical science Volume: 13 ISSN: 1021-7770 ISO Abbreviation: J. Biomed. Sci. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-29 Completed Date: 2007-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421567 Medline TA: J Biomed Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 721-30 Citation Subset: IM |
Affiliation:
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Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan, Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins E / deficiency Carbon Monoxide / physiology* Carotid Artery, Common / physiopathology* Fibrin / metabolism Fibrinogen / metabolism Heme Oxygenase-1 / genetics, physiology* Hypercholesterolemia / physiopathology* Inflammation / metabolism, physiopathology Male Mice Muscle Development Muscle, Smooth, Vascular / growth & development Plasminogen Activator Inhibitor 1 / metabolism Thrombosis / physiopathology* Transduction, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins E; 0/Plasminogen Activator Inhibitor 1; 630-08-0/Carbon Monoxide; 9001-31-4/Fibrin; 9001-32-5/Fibrinogen; EC 1.14.99.3/Heme Oxygenase-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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