| Carbon monoxide disposition in the perfused rat liver. | |
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MedLine Citation:
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PMID: 10484400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple method for determining carbon monoxide (CO) disposition in the rat liver perfused with erythrocyte-free buffer was developed. Wash-in experiments were performed with buffer containing tracer quantities of [14C]sucrose and 3H2O and equilibrated with CO. Outflow samples were collected into tubes containing human erythrocytes, which avidly bind CO. Outflow curves were analyzed using compartmental models. Fractional recovery of CO was 1.07 +/- 0. 17, and the apparent volume of distribution was 1.37 +/- 0.30 ml/g of liver (n = 8). A flow-limited model fitted the data most effectively, although estimates of the permeability-to-surface area product were attempted using a barrier-limited model. This technique will facilitate investigation of the effects of disease on gaseous substrate disposition in perfused organs. |
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Authors:
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D G le Couteur; Z L Yin; L P Rivory; A J McLean |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 277 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-10-28 Completed Date: 1999-10-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G725-30 Citation Subset: IM |
Affiliation:
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Canberra Clinical School of the Sydney University, The Canberra Hospital, Garran, Australian Capital Territory 2605, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Monoxide / blood, metabolism* Erythrocytes / metabolism Humans Liver / metabolism* Male Models, Biological Perfusion Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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630-08-0/Carbon Monoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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