Document Detail


Carbon isotope analysis of acetaldehyde emitted from leaves following mechanical stress and anoxia.
MedLine Citation:
PMID:  19538397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the emission of acetaldehyde from plants into the atmosphere following biotic and abiotic stresses may significantly impact air quality and climate, its metabolic origin(s) remains uncertain. We investigated the pathway(s) responsible for the production of acetaldehyde in plants by studying variations in the stable carbon isotope composition of acetaldehyde emitted during leaf anoxia or following mechanical stress. Under an anoxic environment, C3 leaves produced acetaldehyde during ethanolic fermentation with a similar carbon isotopic composition to C3 bulk biomass. In contrast, the initial emission burst following mechanical wounding was 5-12 per thousand more depleted in (13)C than emissions under anoxia. Due to a large kinetic isotope effect during pyruvate decarboxylation catalysed by pyruvate dehydrogenase, acetyl-CoA and its biosynthetic products such as fatty acids are also depleted in (13)C relative to bulk biomass. It is well known that leaf wounding stimulates the release of large quantities of fatty acids from membranes, as well as the accumulation of reactive oxygen species (ROS). We suggest that, following leaf wounding, acetaldehyde depleted in (13)C is produced from fatty acid peroxidation reactions initiated by the accumulation of ROS. However, a variety of other pathways could also explain our results, including the conversion of acetyl-CoA to acetaldehyde by the esterase activity of aldehyde dehydrogenase.
Authors:
K Jardine; T Karl; M Lerdau; P Harley; A Guenther; J E Mak
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant biology (Stuttgart, Germany)     Volume:  11     ISSN:  1435-8603     ISO Abbreviation:  Plant Biol (Stuttg)     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-08-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101148926     Medline TA:  Plant Biol (Stuttg)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  591-7     Citation Subset:  IM    
Affiliation:
School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, USA. jardine@b2science.org
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MeSH Terms
Descriptor/Qualifier:
Acetaldehyde / analysis,  metabolism*
Carbon Isotopes / analysis*
Cell Hypoxia / physiology*
Plant Leaves / metabolism*
Populus / metabolism
Stress, Mechanical*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 75-07-0/Acetaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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