Document Detail


Carbon and Nitrogen Provisions Alter the Metabolic Flux in Developing Soybean Embryos.
MedLine Citation:
PMID:  23314943     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Soybean seeds store significant amounts of their biomass as protein, levels of which reflect the carbon and nitrogen received by the developing embryo. The relationship between carbon and nitrogen supply during filling and seed composition was examined through a series of embryo culturing experiments. Three distinct ratios of carbon to nitrogen supply (C:N ratio) were further explored through metabolic flux analysis. Labeling experiments utilizing [U-13C5]-glutamine, [U-13C4]-asparagine, and [1,2-13C2]-glucose were performed to assess embryo metabolism under altered feeding conditions and to create corresponding flux maps. Additionally, [U-14C12]-sucrose, [U-14C6]-glucose, [U-14C5]-glutamine, and [U-14C4]-asparagine were used to monitor differences in carbon allocation. The analyses revealed that: i) protein concentration as a percentage of total soybean embryo biomass coincided with C:N ratio; ii) altered nitrogen supply did not dramatically impact relative amino acid or storage protein subunit profiles; and iii) glutamine supply contributed 10 to 23% of the carbon for biomass production, including 9 to 19% of carbon to fatty acid biosynthesis and 32 to 46% of carbon to amino acids. Seed metabolism accommodated different levels of protein biosynthesis while maintaining a consistent rate of dry weight accumulation. Flux through ATP-citrate lyase, combined with malic enzyme activity, contributed significantly to acetyl CoA production. These fluxes changed with plastidic pyruvate kinase to maintain a supply of pyruvate for amino and fatty acids. The flux maps were independently validated by nitrogen balancing and highlight the robustness of primary metabolism.
Authors:
Doug K Allen; Jamey D Young
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-11
Journal Detail:
Title:  Plant physiology     Volume:  -     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 USDA-ARS/Donald Danforth Plant Science Center;
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