Document Detail

Carbohydrates and purines in underperfused hearts, protected by ischemic preconditioning.
MedLine Citation:
PMID:  9515044     Owner:  NLM     Status:  MEDLINE    
Few results, and those controversial, have been published on ischemic preconditioning followed by low-flow ischemia. The aim of this study was to assess whether ischemic preconditioning: (1) confers protection against severe underperfusion; and (2) is mediated by mobilization of proglycogen, resulting in increased anaerobic glycolysis and reduced myocardial injury. Isolated rat hearts were retrogradely perfused and subjected to either 25 min low-flow ischemia (0.6 ml/min) followed by 30 min reperfusion (IC; n=5), or the same protocol preceded by two cycles of 5 min no-flow ischemia and 5 min reperfusion (PC; n=7). Additionally, hearts (n=52) were freeze-clamped at different time points throughout the protocol. Preconditioning improved functional recovery (developed force X heart rate in PC hearts: 54 v 21% in IC hearts; P<0.01) and reduced ischemic damage (cumulative release of creatine kinase during reperfusion: 93 v 215 micro/g dry weight; P<0.05). During ischemia and reperfusion, release of adenosine and the sum of purines was smaller in PC hearts (P<0.05), while lactate release was similar in the two groups. PC reduced both macroglycogen and proglycogen by c. 60% (P<0.01) resulting in constant glycogen levels during low-flow ischemia. In contrast, in IC hearts, both fractions decreased by c. 60% during underperfusion (P<0.01). These results demonstrate that: (1) ischemic preconditioning reduces injury due to severe flow reduction; and (2) preconditioning reduced glycogenolysis without affecting anaerobic glycolysis, suggesting increased glucose uptake.
R de Jonge; S Bradamante; L Speleman; J Willem de Jong
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  30     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-05-01     Completed Date:  1998-05-01     Revised Date:  2010-01-15    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  699-708     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press Limited
Cardiochemical Laboratory, ThoraxCenter, Erasmus University Rotterdam, The Netherlands.
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MeSH Terms
Adenosine Triphosphate / metabolism
Carbohydrate Metabolism*
Glucose / metabolism
Glycogen / metabolism
Ischemic Preconditioning, Myocardial*
Lactic Acid / metabolism
Myocardial Contraction
Myocardial Reperfusion Injury / metabolism*,  physiopathology,  prevention & control*
Myocardium / metabolism*,  pathology
Phosphocreatine / metabolism
Purines / metabolism*
Rats, Wistar
Reg. No./Substance:
0/Purines; 50-21-5/Lactic Acid; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 67-07-2/Phosphocreatine; 9005-79-2/Glycogen

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