Document Detail


Carbohydrate-to-fat ratio affects food intake and body weight in Wistar rats.
MedLine Citation:
PMID:  20558837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the study was to evaluate the impact of carbohydrate-to-fat ratio on body weight and appetite regulation in Wistar rats. Twenty-four Wistar rats were randomized to three dietary groups (n = 8): normal carbohydrate diet (NC), low-carbohydrate diet (LC) and high-carbohydrate diet (HC) for 12 weeks. Body weight and food intake were recorded. Circulating leptin and insulin levels were measured by radioimmunoassay method. The expression levels of leptin receptor, insulin receptor, orexin, neuropeptide Y (NPY), agouti-related protein (AgRP) and melanocortin-4 receptor (MC-4R) in the hypothalamus were also measured by realtime polymerase chain reaction (PCR). In the LC group, food intake reduced while body weight increased significantly compared with the NC and HC groups. Plasma leptin levels increased in the LC (18.5 +/- 8.2 ng/mL) group compared with the NC (8.6 +/- 3.8 ng/mL, P < 0.001) and HC (6.6 +/- 1.9 ng/mL, P < 0.001) groups. Realtime reverse transcription-PCR revealed a decrease in the hypothalamic expression level of only leptin receptor in the LC (0.764, 0.471-4.648 copy/mL) and HC (0.357, 0.129-0.781 copy/mL) groups compared with the NC (1.323, 0.616-2.392 copy/mL; P = 0.01) group, and that there was no significant change in those of insulin receptor, AgRP, Orexin, NPY and MC-4R. Low-carbohydrate, high-fat diet raised body weight, which led to a rising of circulating leptin levels and a reduced expression of leptin receptor in the hypothalamus.
Authors:
Ren-Ying Xu; Yan-Ping Wan; Qing-Ya Tang; Jiang Wu; Wei Cai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  235     ISSN:  1535-3699     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-18     Completed Date:  2010-07-09     Revised Date:  2010-07-29    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  England    
Other Details:
Languages:  eng     Pagination:  833-8     Citation Subset:  IM    
Affiliation:
Department of Clinical Nutrition, Ren Ji Hospital and Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Agouti-Related Protein / analysis
Animals
Appetite Regulation / physiology*
Body Weight / physiology*
Dietary Carbohydrates / pharmacology*
Dietary Fats / pharmacology*
Eating / physiology
Hypothalamus / chemistry
Insulin / blood
Intracellular Signaling Peptides and Proteins / analysis
Leptin / blood
Male
Neuropeptide Y / analysis
Neuropeptides / analysis
Polymerase Chain Reaction
Rats
Rats, Wistar / metabolism,  physiology
Receptor, Insulin / analysis
Receptor, Melanocortin, Type 4 / analysis
Receptors, Leptin / analysis
Chemical
Reg. No./Substance:
0/Agouti-Related Protein; 0/Dietary Carbohydrates; 0/Dietary Fats; 0/Intracellular Signaling Peptides and Proteins; 0/Leptin; 0/Neuropeptide Y; 0/Neuropeptides; 0/Receptor, Melanocortin, Type 4; 0/Receptors, Leptin; 0/orexins; 11061-68-0/Insulin; EC 2.7.10.1/Receptor, Insulin

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