Document Detail

Carbohydrate structures of hen ovomucoid. A mass spectrometric analysis.
MedLine Citation:
PMID:  6852268     Owner:  NLM     Status:  MEDLINE    
The apparently homogenous N-glycosidically-linked glycans 1, 7, 11 and 14 released by hydrazinolysis from hen ovomucoid were analysed by fast atom bombardment and electron-impact mass spectrometry after reduction and permethylation. The spectra support the primary structures established independently [FEBS Letters (1983) 152, 145-152] using methylation analysis, partial acid hydrolysis and 500 MHz 1H NMR spectroscopy. In addition to the major constituents present in fractions 1, 7, 11 and 14, four minor components not detected by other methods could be characterized with the aid of the mass spectrometry data as: Man2GlcNAcGlcNAc-o1, GlcNAc4Man3GlcNAc-o1, GlcNAc6Man3GlcNAc-o1 and GalGlcNAc6-Man3GlcNAc-o1. Our results show that the physical techniques used provide valuable data on the structure of complex glycans. In addition they can be employed to ascertain the homogeneity of the compounds examined as well as to detect trace amounts of homologs that might not be noticed by other methods.
H Egge; J Peter-Katalinić; J Paz-Parente; G Strecker; J Montreuil; B Fournet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  156     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-07-29     Completed Date:  1983-07-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  357-62     Citation Subset:  IM    
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MeSH Terms
Carbohydrates / isolation & purification*
Chemical Phenomena
Egg Proteins*
Mass Spectrometry
Reg. No./Substance:
0/Carbohydrates; 0/Egg Proteins; 37281-36-0/Ovomucin

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