Document Detail


Carbohydrate polymers for nonviral nucleic acid delivery.
MedLine Citation:
PMID:  21504102     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carbohydrates have been investigated and developed as delivery vehicles for shuttling nucleic acids into cells. In this review, we present the state of the art in carbohydrate-based polymeric vehicles for nucleic acid delivery, with the focus on the recent successes in preclinical models, both in vitro and in vivo. Polymeric scaffolds based on the natural polysaccharides chitosan, hyaluronan, pullulan, dextran, and schizophyllan each have unique properties and potential for modification, and these results are discussed with the focus on facile synthetic routes and favorable performance in biological systems. Many of these carbohydrates have been used to develop alternative types of biomaterials for nucleic acid delivery to typical polyplexes, and these novel materials are discussed. Also presented are polymeric vehicles that incorporate copolymerized carbohydrates into polymer backbones based on polyethylenimine and polylysine and their effect on transfection and biocompatibility. Unique scaffolds, such as clusters and polymers based on cyclodextrin (CD), are also discussed, with the focus on recent successes in vivo and in the clinic. These results are presented with the emphasis on the role of carbohydrate and charge on transfection. Use of carbohydrates as molecular recognition ligands for cell-type specific delivery is also briefly reviewed. We contend that carbohydrates have contributed significantly to progress in the field of non-viral DNA delivery, and these new discoveries are impactful for developing new vehicles and materials for treatment of human disease.
Authors:
Antons Sizovs; Patrick M McLendon; Sathya Srinivasachari; Theresa M Reineke
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Topics in current chemistry     Volume:  296     ISSN:  0340-1022     ISO Abbreviation:  Top Curr Chem     Publication Date:  2010  
Date Detail:
Created Date:  2011-04-19     Completed Date:  2011-05-04     Revised Date:  2014-03-21    
Medline Journal Info:
Nlm Unique ID:  0432204     Medline TA:  Top Curr Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  131-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carbohydrates / chemistry*
Humans
Nucleic Acids / genetics*
Polymers / chemistry,  metabolism*
Transfection / methods*
Grant Support
ID/Acronym/Agency:
DP2 OD006669/OD/NIH HHS
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Nucleic Acids; 0/Polymers

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