| Carbohydrate moieties of procollagen: incorporation of isotopically labeled mannose and glucosamine into propeptides of procollagen secreted by matrix-free chick embryo tendon cells. | |
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MedLine Citation:
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PMID: 1061125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells obtained from chick embryo tendons incorporate isotopically labeled glucosamine and mannose into the pro-alpha1 and pro-alpha2 chains of procollagen as judged by sodium dodecyl sulfate-gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The label was further localized to the propeptides of pro-alpha1 and pro-alpha2 by its chromatographic behavior after digestion with bacterial collagenase or alpha-chymotrypsin. Carbohydrate analysis of isolated pro-alpha chains showed the presence of labeled galactosamine in addition to mannose and glucosamine. Resistance to mild alkaline hydrolysis suggested that greater than 90% of the oligosaccharide units are not linked to the propeptide backbone by either serine or threonine. |
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Authors:
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C C Clark; N A Kefalides |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 73 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1976 Jan |
Date Detail:
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Created Date: 1976-04-01 Completed Date: 1976-04-01 Revised Date: 2010-09-03 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 34-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chick Embryo Chromatography, Gel Collagen / biosynthesis*, secretion Electrophoresis, Polyacrylamide Gel Glucosamine / metabolism* Hydrolysis Mannose / metabolism* Protein Precursors / biosynthesis*, secretion Serine / metabolism Threonine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Protein Precursors; 31103-86-3/Mannose; 3416-24-8/Glucosamine; 56-45-1/Serine; 72-19-5/Threonine; 9007-34-5/Collagen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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