Document Detail


Carbohydrate moieties of the interstitial and glandular tissues of the amphibian Pleurodeles waltl testis shown by lectin histochemistry.
MedLine Citation:
PMID:  11215767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amphibian testis is a useful model because of its zonal organisation in lobules, distributed along the cephalocaudal axis, each containing a unique germ cell type. Sperm empty lobules form the so-called glandular tissue at the posterior region of the gonad. Androgen production is limited to the cells of the interstitial tissue surrounding lobules with spermatozoa bundles and to the cells of the glandular tissue. In this work, we have studied the distribution of terminal carbohydrate moieties of N- and O-linked oligosaccharides in the interstitial and glandular tissue of the Pleurodeles waltl testis, by means of 14 lectins combined with chemical and enzymatic deglycosylation pretreatment. Some differences in glycan composition between the interstitial and the glandular tissue have been detected. Thus in both tissues, N-linked oligosaccharides contained mannose, Gal(beta1,4)GlcNAc, and Neu5Ac(alpha2,3)Gal(beta1,4)GlcNAc, while O-linked oligosaccharides contained Con A-positive mannose, Gal(beta1,3)GalNAc, Gal(beta1,4)GlcNAc, Neu5Ac(alpha2,3)Gal(beta1,4)GlcNAc, and WGA-positive GlcNAc. Fucose was also detected in both tissues. However, GlcNAc on N-linked oligosaccharides and GalNAc and Neu5Ac(alpha2,6)Gal/GalNAc on both N- and O-linked oligosaccharides were found only in the interstitial tissue. As glandular tissue cells arise from the innermost cells of interstitial tissue that surround lobules, the differences in the glycan composition of interstitial and glandular tissue shown in this work may be related to the start of androgen synthesis when steroid hormone (SH)-secreting cells develop.
Authors:
F J Sáez; J F Madrid; R Aparicio; F Hernández; E Alonso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of anatomy     Volume:  198     ISSN:  0021-8782     ISO Abbreviation:  J. Anat.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-02-15     Completed Date:  2001-03-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0137162     Medline TA:  J Anat     Country:  England    
Other Details:
Languages:  eng     Pagination:  47-56     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Morphological Sciences, University of the Basque Country, Leioa (Vizcaya), Spain. gcpsacrf@lg.ehu.es
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MeSH Terms
Descriptor/Qualifier:
Androgens / biosynthesis
Angiography, Digital Subtraction
Animals
Concanavalin A
Fucose / analysis
Histocytochemistry / methods
Lectins
Leydig Cells / chemistry
Male
Mannose-Binding Lectins*
Oligosaccharides / analysis*
Peanut Agglutinin
Plant Lectins*
Pleurodeles / metabolism*
Testis / chemistry*
Wheat Germ Agglutinins
Chemical
Reg. No./Substance:
0/Androgens; 0/Lectins; 0/Limax flavus agglutinin; 0/Mannose-Binding Lectins; 0/Oligosaccharides; 0/Peanut Agglutinin; 0/Plant Lectins; 0/Wheat Germ Agglutinins; 0/dolichos biflorus agglutinin; 0/snowdrop lectin; 11028-71-0/Concanavalin A; 3713-31-3/Fucose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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