| CarF mediates signaling by singlet oxygen, generated via photoexcited protoporphyrin IX, in Myxococcus xanthus light-induced carotenogenesis. | |
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MedLine Citation:
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PMID: 22267513 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Blue light triggers carotenogenesis in the non-phototrophic bacterium Myxococcus xanthus by inducing inactivation of an anti-σ factor, CarR, and the consequent liberation of the cognate extracytoplasmic function (ECF) σ, CarQ. CarF, the protein implicated earliest in the response to light, does not resemble any known photoreceptor. It interacts physically with CarR and is required for its light-driven inactivation but the mechanism is unknown. Blue light sensing in M. xanthus has been attributed to the heme precursor, protoporphyrin IX (PPIX), which can generate the highly reactive singlet oxygen species ((1)O(2)) by energy transfer to oxygen. However, (1)O(2) involvement in M. xanthus light-induced carotenogenesis remains to be established. Here, we present genetic evidence on the involvement of PPIX as well as (1)O(2) inlight-induced carotenogenesis in M. xanthus, and on how these are linked to CarF in the signal transduction pathway. Response to light was examined in carF-bearing or carF-deficient M. xanthus strains lacking endogenous PPIX due to deletion of hemB, or accumulating PPIX due to deletion of hemH (hemB and hemH are early- and late-acting heme biosynthesis genes, respectively). This demonstrated that light-induction of the CarQ-dependent promoter, P(QRS), correlated directly with cellular PPIX levels. Furthermore, we show that P(QRS) activation is triggered by (1)O(2) and is inhibited by exogenously supplied hemin, and that CarF is essential for the action of (1)O(2). Thus, our findings indicate that blue light interaction with PPIX generates (1)O(2), which must be transmitted via CarF to trigger the transcriptional response underlying light-induced carotenogenesis in M. xanthus. |
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Authors:
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Marisa Galbis-Martínez; S Padmanabhan; Francisco J Murillo; Montserrat Elías-Arnanz |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: Journal of bacteriology Volume: - ISSN: 1098-5530 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Departamento de Genética y Microbiología, Área de Genética (Unidad Asociada al IQFR-CSIC), Facultad de Biología, Universidad de Murcia, Murcia 30100, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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