Document Detail


Capturing directed molecular motion in the nuclear pore complex of live cells.
MedLine Citation:
PMID:  22665783     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. Intrinsically disordered nucleoporins (Nups) form a selective filter inside the NPC, taking a central role in the vital nucleocytoplasmic transport mechanism. How such intricate meshwork relates to function and gives rise to a transport mechanism is still unclear. Here we set out to tackle this issue in intact cells by an established combination of fluorescence correlation spectroscopy and real-time tracking of the center of mass of single NPCs. We find the dynamics of nucleoporin Nup153 to be regulated so as to produce rapid, discrete exchange between two separate positions within the NPC. A similar behavior is also observed for both karyopherinβ1 transport-receptor and cargoes destined to nuclear import. Thus, we argue that directed Nup-mediated molecular motion may represent an intrinsic feature of the overall selective gating through intact NPCs.
Authors:
Francesco Cardarelli; Luca Lanzano; Enrico Gratton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-06-04
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-20     Completed Date:  2012-09-10     Revised Date:  2013-07-30    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9863-8     Citation Subset:  IM    
Affiliation:
Center for Nanotechnology Innovation at National Enterprise for nanoScience and nanoTechnology, Istituto Italiano di Tecnologia, 56127 Pisa, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cricetinae
Cricetulus
Green Fluorescent Proteins / metabolism
Nuclear Pore / metabolism*
Nuclear Pore Complex Proteins / metabolism
Protein Transport
Spectrometry, Fluorescence
Grant Support
ID/Acronym/Agency:
5P41RR003155/RR/NCRR NIH HHS; 5P50 GM076516/GM/NIGMS NIH HHS; 8P41GM103540/GM/NIGMS NIH HHS; P30 CA062203/CA/NCI NIH HHS; P41 GM103540/GM/NIGMS NIH HHS; P41 RR003155/RR/NCRR NIH HHS; P50 GM076516/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Nuclear Pore Complex Proteins; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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