| Capture of plasma membrane fragments from target cells by trogocytosis requires signaling in T cells but not in B cells. | |
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MedLine Citation:
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PMID: 18381976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Upon recognition of their respective cellular partners, T and B cells acquire their antigens by a process of membrane capture called trogocytosis. Here, we report that various inhibitors of actin polymerization or of kinases involved in intracellular signaling partially or fully inhibited trogocytosis by CD8(+) and CD4(+) T cells, whereas they had no effect on trogocytosis by B cells. Similarly, trogocytosis by T cells was inhibited at 4 degrees C, whereas in B cells it was independent of temperature, indicating that trogocytosis by B cells does not rely on active processes. By contrast, most inhibitors we tested impaired both T-cell and B-cell activation. The differential effect of inhibitors on T-cell and B-cell trogocytosis was not due to the higher affinity of the B-cell receptor for its cognate antigen compared with the affinity of the T-cell receptor for its own antigen, but it correlated tightly with the abilities of T cells and B cells to form conjugates with their target cells in the presence of inhibitors. Trogocytosis thus has different requirements in different cell types. Moreover, the capture of membrane antigen by B cells is identified as a novel signaling-independent event of B-cell biology. |
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Authors:
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Anne Aucher; Eddy Magdeleine; Etienne Joly; Denis Hudrisier |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-01 |
Journal Detail:
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Title: Blood Volume: 111 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-06-11 Completed Date: 2008-07-15 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 5621-8 Citation Subset: AIM; IM |
Affiliation:
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Institut de Pharmacologie et de Biologie Structurale, Centre National de Recherche Scientifique, Unité Mixte de Recherche 5089, Toulouse, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen Presentation / immunology B-Lymphocytes / cytology*, immunology, metabolism* CD8-Positive T-Lymphocytes / cytology*, immunology, metabolism* Cell Line Cell Membrane / immunology, metabolism Enzyme Inhibitors / pharmacology Humans Intracellular Signaling Peptides and Proteins / antagonists & inhibitors, metabolism Lymphocyte Activation Mice Mice, Transgenic Microfilaments / immunology Phosphatidylinositol 3-Kinases / antagonists & inhibitors, metabolism Protein-Tyrosine Kinases / antagonists & inhibitors, metabolism Receptors, Antigen, B-Cell / metabolism Receptors, Antigen, T-Cell / metabolism Signal Transduction / immunology* src-Family Kinases / antagonists & inhibitors, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Intracellular Signaling Peptides and Proteins; 0/Receptors, Antigen, B-Cell; 0/Receptors, Antigen, T-Cell; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Syk kinase; EC 2.7.10.2/src-Family Kinases |
| Comments/Corrections | |
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