Document Detail


Capture of plasma membrane fragments from target cells by trogocytosis requires signaling in T cells but not in B cells.
MedLine Citation:
PMID:  18381976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Upon recognition of their respective cellular partners, T and B cells acquire their antigens by a process of membrane capture called trogocytosis. Here, we report that various inhibitors of actin polymerization or of kinases involved in intracellular signaling partially or fully inhibited trogocytosis by CD8(+) and CD4(+) T cells, whereas they had no effect on trogocytosis by B cells. Similarly, trogocytosis by T cells was inhibited at 4 degrees C, whereas in B cells it was independent of temperature, indicating that trogocytosis by B cells does not rely on active processes. By contrast, most inhibitors we tested impaired both T-cell and B-cell activation. The differential effect of inhibitors on T-cell and B-cell trogocytosis was not due to the higher affinity of the B-cell receptor for its cognate antigen compared with the affinity of the T-cell receptor for its own antigen, but it correlated tightly with the abilities of T cells and B cells to form conjugates with their target cells in the presence of inhibitors. Trogocytosis thus has different requirements in different cell types. Moreover, the capture of membrane antigen by B cells is identified as a novel signaling-independent event of B-cell biology.
Authors:
Anne Aucher; Eddy Magdeleine; Etienne Joly; Denis Hudrisier
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-01
Journal Detail:
Title:  Blood     Volume:  111     ISSN:  1528-0020     ISO Abbreviation:  Blood     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-11     Completed Date:  2008-07-15     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5621-8     Citation Subset:  AIM; IM    
Affiliation:
Institut de Pharmacologie et de Biologie Structurale, Centre National de Recherche Scientifique, Unité Mixte de Recherche 5089, Toulouse, France.
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / immunology
Animals
Antigen Presentation / immunology
B-Lymphocytes / cytology*,  immunology,  metabolism*
CD8-Positive T-Lymphocytes / cytology*,  immunology,  metabolism*
Cell Line
Cell Membrane / immunology,  metabolism
Enzyme Inhibitors / pharmacology
Humans
Intracellular Signaling Peptides and Proteins / antagonists & inhibitors,  metabolism
Lymphocyte Activation
Mice
Mice, Transgenic
Phosphatidylinositol 3-Kinases / antagonists & inhibitors,  metabolism
Protein-Tyrosine Kinases / antagonists & inhibitors,  metabolism
Receptors, Antigen, B-Cell / metabolism
Receptors, Antigen, T-Cell / metabolism
Signal Transduction / immunology*
src-Family Kinases / antagonists & inhibitors,  metabolism
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Intracellular Signaling Peptides and Proteins; 0/Receptors, Antigen, B-Cell; 0/Receptors, Antigen, T-Cell; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Syk kinase; EC 2.7.10.2/src-Family Kinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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