Document Detail


Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array.
MedLine Citation:
PMID:  22983436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Early detection and isolation of circulating tumor cells (CTC) can enable better prognosis for cancer patients. A Hele-Shaw device with aptamer functionalized glass beads is designed, modeled, and fabricated to efficiently isolate cancer cells from a cellular mixture. The glass beads are functionalized with anti-epidermal growth factor receptor (EGFR) aptamer and sit in ordered array of pits in polydimethylsiloxane (PDMS) channel. A PDMS encapsulation is then used to cover the channel and to flow through cell solution. The beads capture cancer cells from flowing solution depicting high selectivity. The cell-bound glass beads are then re-suspended from the device surface followed by the release of 92% cells from glass beads using combination of soft shaking and anti-sense RNA. This approach ensures that the cells remain in native state and undisturbed during capture, isolation and elution for post-analysis. The use of highly selective anti-EGFR aptamer with the glass beads in an array and subsequent release of cells with antisense molecules provide multiple levels of binding and release opportunities that can help in defining new classes of CTC enumeration devices.
Authors:
Yuan Wan; Yaling Liu; Peter B Allen; Waseem Asghar; M Arif Iftakher Mahmood; Jifu Tan; Holli Duhon; Young-tae Kim; Andrew D Ellington; Samir M Iqbal
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-17     Completed Date:  2013-03-14     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  4693-701     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aptamers, Nucleotide / genetics,  metabolism*
Base Sequence
Blood Specimen Collection
Cell Separation / instrumentation*
Dimethylpolysiloxanes / chemistry
Equipment Design
Erythrocytes / cytology
Glass / chemistry*
Humans
Mechanical Processes
Microspheres*
Molecular Sequence Data
Neoplastic Cells, Circulating / pathology*
Receptor, Epidermal Growth Factor / metabolism
Grant Support
ID/Acronym/Agency:
1-R21-HG005763-01/HG/NHGRI NIH HHS; 5R01CA119388-05/CA/NCI NIH HHS; EB009786/EB/NIBIB NIH HHS; GM095280/GM/NIGMS NIH HHS; R01 GM094933/GM/NIGMS NIH HHS; R21 HG005763/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 0/Dimethylpolysiloxanes; 63148-62-9/baysilon; EC 2.7.10.1/Receptor, Epidermal Growth Factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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