Document Detail


Capture and culturing of living cells on microstructured DNA substrates.
MedLine Citation:
PMID:  20730825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modular system for the DNA-directed immobilization of antibodies was applied to capture living cells on microstructured DNA surfaces. It is demonstrated in two different set-ups, static incubation and hydrodynamic flow, that this approach is well suited for specific capture and selection of cells from culture medium. The adhered cells show intact morphology and they can be cultivated to grow to dense monolayers, restricted to the lateral dimensions of DNA spots on the surface. Owing to the modularity of surface biofunctionalization, the system can readily be configured to serve as a matrix for adhesion and growth of different cells, as demonstrated by specific binding of human embryonic kidney cells (HEK293) and Hodgkin lymphoma L540cy cells onto patches bearing appropriate recognition moieties inside a microfluidic channel. We therefore anticipate that the systems described here should be useful for fundamental research in cell biology or applications in biomedical diagnostics, drug screening, and nanobiotechnology.
Authors:
Stephanie Reisewitz; Hendrik Schroeder; Nuria Tort; Katie A Edwards; Antje J Baeumner; Christof M Niemeyer
Related Documents :
19405725 - Delivery of optical contrast agents using triton-x100, part 1: reversible permeabilizat...
10504215 - Targeted recombinant aequorins: tools for monitoring [ca2+] in the various compartments...
1637285 - What is actually stained by rose bengal?
12720355 - Scanning electrochemical microscopy-based drug sensitivity test for a cell culture inte...
22204275 - Baicalein mediates inhibition of migration and invasiveness of skin carcinoma through e...
8725505 - Effects of corticosteroid therapy in non-allergic rhinosinusitis.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  6     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2162-8     Citation Subset:  IM    
Affiliation:
Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto Hahn Str. 6, 44227 Dortmund, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies / chemistry
Cell Adhesion
Cell Line
Cell Line, Tumor
Cytological Techniques*
DNA / chemistry*
Dendrimers / chemistry
Humans
Chemical
Reg. No./Substance:
0/Antibodies; 0/Dendrimers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Group IV nanoparticles: synthesis, properties, and biological applications.
Next Document:  First-layer effect in graphene-enhanced Raman scattering.