| Capsaicin induces apoptosis in SCC-4 human tongue cancer cells through mitochondria-dependent and -independent pathways. | |
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MedLine Citation:
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PMID: 20925121 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Although there have been advances in the fields of surgery, radiotherapy, and chemotherapy of tongue cancer, the cure rates are still not substantially satisfactory. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is the major pungent ingredient of hot chili pepper and has been reported to have an antitumor effect on many human cancer cell types. The molecular mechanisms of the antitumor effect of capsaicin are not yet completely understood. Herein, we investigated whether capsaicin induces apoptosis in human tongue cancer cells. Capsaicin decreased the percentage of viable cells in a dose-dependent manner in human tongue cancer SCC-4 cells. In addition, capsaicin produced DNA fragmentation, decreased the DNA contents (sub-G1 phase), and induced G0/G1 phase arrest in SCC-4 cells. We demonstrated that capsaicin-induced apoptosis is associated with an increase in reactive oxygen species and Ca(2+) generations and a disruption of the mitochondrial transmenbrane potential (ΔΨ(m)) . Treatment with capsaicin induced a dramatic increase in caspase-3 and -9 activities, as assessed by flow cytometric methods. A possible mechanism of capsaicin-induced apoptosis is involved in the activation of caspase-3 (one of the apoptosis-executing enzyme). Confocal laser microscope examination also showed that capsaicin induced the releases of AIF, ATF-4, and GADD153 from mitochondria of SCC-4 cells. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012. |
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Authors:
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Siu-Wan Ip; Sheng-Hui Lan; An-Cheng Huang; Jai-Sing Yang; Ya-Yin Chen; Hui-Ying Huang; Zen-Pin Lin; Yuan-Man Hsu; Mei-Due Yang; Chang-Fang Chiu; Jing-Gung Chung |
Publication Detail:
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Type: Journal Article Date: 2010-10-05 |
Journal Detail:
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Title: Environmental toxicology Volume: 27 ISSN: 1522-7278 ISO Abbreviation: Environ. Toxicol. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100885357 Medline TA: Environ Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 332-41 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley Periodicals, Inc. |
Affiliation:
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Department of Nutrition, China Medical University, Taichung 404, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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