| Capsaicin induced apoptosis of B16-F10 melanoma cells through down-regulation of Bcl-2. | |
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MedLine Citation:
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PMID: 17306913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Capsaicin (8-methyl-N-vanillyl-6-nonenamide), a pungent ingredient of hot chili peppers, has been reported to possess substantial anticarcinogenic and antimutagenic activities. In the present study, we investigated the effect of capsaicin on induction of apoptosis in highly metastatic B16-F10 murine melanoma cells. Capsaicin inhibited growth of B16-F10 cells in a concentration-dependent manner. Proapoptotic effect of capsaicin was evidenced by nuclear condensation, internucleosomal DNA fragmentation, in situ terminal nick-end labeling of fragmented DNA (TUNEL), and an increased sub G1 fraction. Treatment of B16-F10 cells with capsaicin caused release of mitochondrial cytochrome c, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase in a dose-dependent manner. Furthermore, Bcl-2 expression in the B16-F10 cells was slightly down-regulated by capsaicin treatment. In contrast, there were no alterations in the levels of Bax in capsaicin-treated cells. Collectively, these findings indicate that capsaicin-induces apoptosis of B16-F10 melanoma cells via down-regulation the Bcl-2. |
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Authors:
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Hye-Seung Jun; Taesun Park; Chang Ki Lee; Mi Kyung Kang; Mi Sun Park; Ho Il Kang; Young-Joon Surh; Ok Hee Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-27 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 45 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-03-27 Completed Date: 2007-05-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 708-15 Citation Subset: IM |
Affiliation:
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National Institute of Toxicological Research, Korea Food and Drug Administration, 5 Nokbun-Dong, Eunpyong-Gu, Seoul 122-704, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate Ribose
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metabolism Animals Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects* Capsaicin / pharmacology* Capsicum / chemistry* Caspase 3 / metabolism Cell Line, Tumor Cell Survival Cytochromes c / metabolism DNA Fragmentation Dose-Response Relationship, Drug Down-Regulation* Flow Cytometry Genes, bcl-2 / drug effects Humans In Situ Nick-End Labeling Melanoma Mice Proto-Oncogene Proteins c-bcl-2 / drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Proto-Oncogene Proteins c-bcl-2; 20762-30-5/Adenosine Diphosphate Ribose; 404-86-4/Capsaicin; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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