Document Detail


Capsaicin induced apoptosis of B16-F10 melanoma cells through down-regulation of Bcl-2.
MedLine Citation:
PMID:  17306913     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Capsaicin (8-methyl-N-vanillyl-6-nonenamide), a pungent ingredient of hot chili peppers, has been reported to possess substantial anticarcinogenic and antimutagenic activities. In the present study, we investigated the effect of capsaicin on induction of apoptosis in highly metastatic B16-F10 murine melanoma cells. Capsaicin inhibited growth of B16-F10 cells in a concentration-dependent manner. Proapoptotic effect of capsaicin was evidenced by nuclear condensation, internucleosomal DNA fragmentation, in situ terminal nick-end labeling of fragmented DNA (TUNEL), and an increased sub G1 fraction. Treatment of B16-F10 cells with capsaicin caused release of mitochondrial cytochrome c, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase in a dose-dependent manner. Furthermore, Bcl-2 expression in the B16-F10 cells was slightly down-regulated by capsaicin treatment. In contrast, there were no alterations in the levels of Bax in capsaicin-treated cells. Collectively, these findings indicate that capsaicin-induces apoptosis of B16-F10 melanoma cells via down-regulation the Bcl-2.
Authors:
Hye-Seung Jun; Taesun Park; Chang Ki Lee; Mi Kyung Kang; Mi Sun Park; Ho Il Kang; Young-Joon Surh; Ok Hee Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-27
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  45     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-05-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  708-15     Citation Subset:  IM    
Affiliation:
National Institute of Toxicological Research, Korea Food and Drug Administration, 5 Nokbun-Dong, Eunpyong-Gu, Seoul 122-704, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate Ribose / metabolism
Animals
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Capsaicin / pharmacology*
Capsicum / chemistry*
Caspase 3 / metabolism
Cell Line, Tumor
Cell Survival
Cytochromes c / metabolism
DNA Fragmentation
Dose-Response Relationship, Drug
Down-Regulation*
Flow Cytometry
Genes, bcl-2 / drug effects
Humans
In Situ Nick-End Labeling
Melanoma
Mice
Proto-Oncogene Proteins c-bcl-2 / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Proto-Oncogene Proteins c-bcl-2; 20762-30-5/Adenosine Diphosphate Ribose; 404-86-4/Capsaicin; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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