| Capsaicin mediates cell death in bladder cancer T24 cells through reactive oxygen species production and mitochondrial depolarization. | |
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MedLine Citation:
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PMID: 19592070 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To investigate the effects of capsaicin (CAP) on proliferation of bladder cancer T24 cells in vitro as well as on xenografts in nude mice in vivo. METHODS: T24 cells were assessed for cell viability and apoptosis by 3-(4, 5-dimethylthiazol-2-yl)-3, 5-diphenyltetrazolium bromide assay and flow cytometry analysis after incubation with different concentrations of CAP. To uncover the mechanism by which CAP affected the viability of T24 cells, intracellular production of reactive oxygen species (ROS) and mitochondrial membrane potential were assessed. To study the in vivo effects of CAP, T24 cells were grown as xenografts in nude mice and CAP (5 mg/kg by wt) was subcutaneously injected into nude mice with bladder tumors. RESULTS: CAP decreased the viability of T24 cells in a dose-dependent manner without marked apoptosis. CAP induced ROS production and mitochondrial membrane depolarization, thereby inducing cell death, not apoptosis, in T24 cells at a concentration of 100 microM or higher. Furthermore, these effects of CAP could be reversed by capsazepine, the antagonist of transient receptor potential vanilloid type 1 channel. In vivo experiment showed that CAP significantly slowed the growth of T24 bladder cancer xenografts as measured by size (661.80 +/- 62.03 vs 567.02 +/- 43.94 mm(3); P <.01). CONCLUSIONS: CAP mediates cell death in T24 cells through calcium entry-dependent ROS production and mitochondrial depolarization, and it may have a role in the management of bladder cancer. |
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Authors:
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Zhong-Hua Yang; Xing-Huan Wang; Huai-Peng Wang; Li-Quan Hu; Xin-Min Zheng; Shi-Wen Li |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-09 |
Journal Detail:
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Title: Urology Volume: 75 ISSN: 1527-9995 ISO Abbreviation: Urology Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-09 Completed Date: 2010-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0366151 Medline TA: Urology Country: United States |
Other Details:
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Languages: eng Pagination: 735-41 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Capsaicin / pharmacology* Cell Death / drug effects Cell Proliferation / drug effects Humans Male Mice Mice, Nude Mitochondria / drug effects*, metabolism* Reactive Oxygen Species / metabolism* Tumor Cells, Cultured Urinary Bladder Neoplasms / metabolism*, pathology* |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 404-86-4/Capsaicin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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