| Caprylic acid and medium-chain triglycerides inhibit IL-8 gene transcription in Caco-2 cells: comparison with the potent histone deacetylase inhibitor trichostatin A. | |
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MedLine Citation:
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PMID: 12010777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Medium-chain triglyceride (MCT) is often administered to patients with Crohn's disease (CD) or short-bowel syndrome. However, little is known about the effects of medium-chain fatty acids (MCFAs) and MCT on intestinal inflammation. In this study we examined whether caprylic acid, one of the MCFAs, and MCT suppress IL-8 secretion by differentiated Caco-2 cells. 2. We found for the first time that caprylic acid and MCT suppress IL-8 secretion by Caco-2 cells at the transcriptional level when precultured together for 24 h. We also tried to clarify the mechanism of IL-8 gene inhibition by examining the activation of NF-kappaB and other transcription factors by electrophoretic mobility shift assay (EMSA), and found that caprylic acid did not modulate their activation. 3. The result of dual-luciferase assay using Caco-2 cells transfected with IL-8 promoter/luciferase reporter plasmid revealed that caprylic acid inhibited the activation of IL-8 promoter. 4. Similar results were observed when cells were precultured with the well-known potent histone deacetylase inhibitor trichostatin A (TSA). 5. We examined the state of H4 acetylation in IL-8 promoter using the technique known as chromatin immunoprecipitation (Chr-IP). TSA rapidly induced H4 acetylation in IL-8 promoter chromatin, whereas caprylic acid did not. These results suggest that the inhibition of IL-8 gene transcription induced by caprylic acid and TSA does not necessarily require the marked suppression of transcription factors, and the mechanism of inhibition of IL-8 gene transcription may be different between caprylic acid and TSA. |
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Authors:
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Aihiro Hoshimoto; Yasuo Suzuki; Tatsuro Katsuno; Hiroshi Nakajima; Yasushi Saito |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: British journal of pharmacology Volume: 136 ISSN: 0007-1188 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-05-15 Completed Date: 2002-10-29 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 280-6 Citation Subset: IM |
Affiliation:
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Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, Chiba, Japan. Hossy@intmed02.m.chiba-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Caco-2 Cells Enzyme Inhibitors / pharmacology Histone Deacetylase Inhibitors* Histone Deacetylases / metabolism Humans Hydroxamic Acids / pharmacology* Interleukin-8 / antagonists & inhibitors*, biosynthesis, genetics* Octanoic Acids / pharmacology* Transcription, Genetic / drug effects*, physiology Triglycerides / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 0/Interleukin-8; 0/Octanoic Acids; 0/Triglycerides; 124-07-2/caprylic acid; 58880-19-6/trichostatin A; EC 3.5.1.98/Histone Deacetylases |
| Comments/Corrections | |
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