Document Detail

Capping of immune complexes by sporozoites of Eimeria tenella.
MedLine Citation:
PMID:  3981346     Owner:  NLM     Status:  MEDLINE    
Sporozoites of Eimeria tenella were incubated for 10, 20, or 30 min with parasite-specific monoclonal IgG antibody 3D3II from mice and then rinsed in a Tris-buffered glucose saline solution (TBGS). Some sporozoites were then incubated for 10, 20, or 30 min with ferritin- or colloidal gold-conjugated goat anti-mouse IgG antibody and then fixed in 2.5% glutaraldehyde and prepared for transmission (TEM) or scanning (SEM) electron microscopy. Other sporozoites that had been previously exposed to monoclonal antibody were prefixed with 0.25% glutaraldehyde, incubated with ferritin- or colloidal gold-conjugated anti-mouse IgG antibody and then fixed and prepared for TEM or SEM. Control preparations consisted of sporozoites exposed only to TBGS, monoclonal antibody 3D3II or to ferritin- or colloidal gold-conjugated anti-mouse IgG antibody. Capping of immune complexes occurred only on the surface of those sporozoites exposed to monoclonal antibody 3D3II followed by ferritin- or gold-conjugated antibody. Immune complexes moved laterally and posteriorly on the outer surface of the parasite plasma membrane to form a cap at the posterior end of the sporozoite. Capping did not occur in TBGS controls nor in sporozoites treated with monoclonal antibody 3D3II and prefixed in 0.25% glutaraldehyde before exposure to ferritin- or gold-conjugated antibody. Thus, capping of surface antigens did not occur in the presence of monoclonal 3D3II antibody only, whereas specimens exposed to both monoclonal and ferritin- or colloidal gold-conjugated antibodies were able to cap immune complexes.
C A Speer; R B Wong; J A Blixt; R H Schenkel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of parasitology     Volume:  71     ISSN:  0022-3395     ISO Abbreviation:  J. Parasitol.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-05-02     Completed Date:  1985-05-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  33-42     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal / immunology
Antigen-Antibody Complex
Cell Membrane / immunology
Eimeria / immunology*
Immunologic Capping
Microscopy, Electron
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigen-Antibody Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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