| Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain. | |
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MedLine Citation:
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PMID: 17289036 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Shigella, the causative agent of bacillary dysentery, invades epithelial cells. Upon bacterial-cell contact, the type III bacterial effector IpaA binds to the cytoskeletal protein vinculin to promote actin reorganization required for efficient bacterial uptake. We show that the last 74 C-terminal residues of IpaA (A559) bind to human vinculin (HV) and promotes its association with actin filaments. Polymerisation experiments demonstrated that A559 was sufficient to induce HV-dependent partial capping of the barbed ends of actin filaments. These results suggest that IpaA regulates actin polymerisation/depolymerisation at sites of Shigella invasion by modulating the barbed end capping activity of vinculin. |
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Authors:
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Nalini Ramarao; Christophe Le Clainche; Tina Izard; Raphaëlle Bourdet-Sicard; Elisabeth Ageron; Philippe J Sansonetti; Marie-France Carlier; Guy Tran Van Nhieu |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-02-02 |
Journal Detail:
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Title: FEBS letters Volume: 581 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-27 Completed Date: 2007-04-17 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 853-7 Citation Subset: IM |
Affiliation:
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Unité de Pathogénie Microbienne Moléculaire, Inserm U786, Institut Pasteur, 75724 Paris Cedex 15, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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chemistry,
metabolism* Animals Antigens, Bacterial / chemistry*, genetics, metabolism* Bacterial Proteins / chemistry*, genetics, metabolism* Binding Sites Biopolymers / chemistry, metabolism Humans Kinetics Protein Structure, Tertiary Rabbits Recombinant Proteins / chemistry, genetics, metabolism Sequence Deletion Shigella / genetics, metabolism*, pathogenicity Vinculin / chemistry, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI067949/AI/NIAID NIH HHS; GM71596/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Biopolymers; 0/IpaA protein, Shigella flexneri; 0/Recombinant Proteins; 0/VCL protein, human; 125361-02-6/Vinculin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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