| Capillary zone electrophoretic separation of C14-C18 linear saturated and unsaturated free fatty acids with indirect UV detection. | |
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MedLine Citation:
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PMID: 9384754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The baseline separation of eight saturated and unsaturated fatty acids of commercial interest was achieved in 15 min by counter-electroosmotic flow capillary zone electrophoresis (CZE), using dimethyl-beta-CD as a buffer additive. The background electrolyte consisted of 10 mM Tris-5 mM p-anisate (pH 8.1) and 1 mM dimethyl-beta-CD in a methanol-water (50:50, vol/vol) mixture. The presence of the cyclodextrin (CD) led to differential migration of critical pairs (i.e., fatty acids of common equivalent chain length or positional isomers). The absence of a highly absorbing chromophore moiety was circumvented through the use of p-anisate as a chromogenic co-ion in the carrier electrolyte. The conditions for indirect absorbance detection were optimized by taking into account the detector linearity and the spectral properties of both analytes and chromophore. A number of parameters influencing the resolution were also discussed, including overloading phenomenon and electroosmotic flow velocity modulation. Quantitative aspects of the method were tested through the analysis of three crude vegetable oils. |
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Authors:
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J Collet; P Gareil |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of capillary electrophoresis Volume: 3 ISSN: 1079-5383 ISO Abbreviation: J Capillary Electrophor Publication Date: 1996 Mar-Apr |
Date Detail:
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Created Date: 1997-12-02 Completed Date: 1997-12-02 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 9507610 Medline TA: J Capillary Electrophor Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 77-82 Citation Subset: IM |
Affiliation:
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Laboratoire d'Electrochimie et de Chimie Analytique, Ecole Nationale Supérieure de Chimie de Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Electrophoresis, Capillary / methods Fatty Acids, Nonesterified / isolation & purification* Fatty Acids, Unsaturated / isolation & purification* Indicators and Reagents Plant Oils / analysis Sensitivity and Specificity Spectrophotometry, Ultraviolet / methods |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Fatty Acids, Unsaturated; 0/Indicators and Reagents; 0/Plant Oils |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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