Document Detail


Capillary zone electrophoresis with electrochemical detection--a simple and effective method to analyze oxygen-consuming and peroxide-forming processes.
MedLine Citation:
PMID:  11765078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous biological, chemical and photochemical processes consume molecular oxygen, thereby forming peroxides, hydroperoxides and even hydrogen peroxide. This paper describes a new system for monitoring quasi simultaneously oxygen uptake and peroxide formation. For this purpose capillary electrophoresis with electrochemical detection was connected to a new automated injector that periodically injects a sample of the reaction mixture into the capillary electrophoresis system without any contact with the atmosphere. The device was tested with some pharmacologically relevant reactions such as enzymatic oxidation of glucose catalyzed by glucose oxidase, enzymatic lipid oxidation by lipoxygenase and the photodynamic reaction of the anti-inflammatory drug ketoprofen.
Authors:
A Radschuweit; H H Rüttinger; P Nuhn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  937     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-05-09     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  127-34     Citation Subset:  IM    
Affiliation:
Martin-Luther-Universität Halle-Wittenberg, Institut für Pharmazeutische Chemie, Germany. radschuweit@pharmazie.uni-halle.de
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MeSH Terms
Descriptor/Qualifier:
Electrochemistry / instrumentation*
Electrophoresis, Capillary / methods*
Glucose Oxidase / chemistry
Linoleic Acid / chemistry
Oxygen / chemistry*
Peroxides / chemistry*
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Peroxides; 2197-37-7/Linoleic Acid; 7782-44-7/Oxygen; EC 1.1.3.4/Glucose Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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