| Capillary zone electrophoresis-based cytotoxicity analysis of Caco-2 cells. | |
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MedLine Citation:
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PMID: 22522537 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A capillary zone electrophoresis-based method to evaluate the cytotoxicity of substances to Caco-2 cells was established. The estimation of the injected cell number (500-5000) and the minor effect of injection condition on cytotoxicity determination were investigated. Caco-2 cells the best model of the intestinal absorptive epithelium, were treated with substances and then stained with Trypan Blue and fixed with paraformaldehyde. The treated Caco-2 cells were detected simultaneously at 590 nm and 214 nm, and the absorbance ratio of the two wavelengths (R(590/214) ) can reflect simultaneously the loss of cell membrane integrity and the degradation/leak of intracellular components and indicate the cytotoxicity of substances. The cytotoxicity of the four substances sodium sulfite (Na(2) SO(3) ), methyl mercury (MeHg), paclitaxel (PTX), and cadmium chloride (CdCl(2) ) were determined and compared. There was no obvious cytotoxicity caused by 20 μM Na(2) SO(3) for 24 h treatment, and the toxicity of the other three toxicants was sequenced as: CdCl(2) > MeHg> PTX. The results are in good agreement with the references and the conventional Trypan Blue exclusion counting assay. |
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Authors:
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Lu Zhang; Feng Qu; Meiling Hu; Jinmei Ding; Beilei Lou |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Electrophoresis Volume: 33 ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-04-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 834-40 Citation Subset: IM |
Copyright Information:
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© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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School of Life Science, Beijing Institute of Technology, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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