Document Detail


Capillary zone electrophoresis-based cytotoxicity analysis of Caco-2 cells.
MedLine Citation:
PMID:  22522537     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A capillary zone electrophoresis-based method to evaluate the cytotoxicity of substances to Caco-2 cells was established. The estimation of the injected cell number (500-5000) and the minor effect of injection condition on cytotoxicity determination were investigated. Caco-2 cells the best model of the intestinal absorptive epithelium, were treated with substances and then stained with Trypan Blue and fixed with paraformaldehyde. The treated Caco-2 cells were detected simultaneously at 590 nm and 214 nm, and the absorbance ratio of the two wavelengths (R(590/214) ) can reflect simultaneously the loss of cell membrane integrity and the degradation/leak of intracellular components and indicate the cytotoxicity of substances. The cytotoxicity of the four substances sodium sulfite (Na(2) SO(3) ), methyl mercury (MeHg), paclitaxel (PTX), and cadmium chloride (CdCl(2) ) were determined and compared. There was no obvious cytotoxicity caused by 20 μM Na(2) SO(3) for 24 h treatment, and the toxicity of the other three toxicants was sequenced as: CdCl(2) > MeHg> PTX. The results are in good agreement with the references and the conventional Trypan Blue exclusion counting assay.
Authors:
Lu Zhang; Feng Qu; Meiling Hu; Jinmei Ding; Beilei Lou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  33     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-04-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  834-40     Citation Subset:  IM    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
School of Life Science, Beijing Institute of Technology, Beijing, China.
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