Document Detail


Capillary and venous samples of total creatine kinase are similar after eccentric exercise.
MedLine Citation:
PMID:  21132860     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Circulating creatine kinase (CK) levels are often monitored as an indirect biomarker of muscle damage after resistive exercise. The purpose of the present investigation was to evaluate whether capillary whole-blood sampling, a simpler and less invasive method for obtaining a venous blood sample, would allow for a reliable measurement of total CK compared to venipuncture. Fifteen untrained subjects performed 50 maximal eccentric elbow extensions to induce muscle damage of the biceps brachii. Capillary (fingerstick) and venous whole-blood samples were collected contemporaneously at baseline and again at 24, 48, 72, and 96 hours post-exercise. Using a commercial CK analysis kit with a protocol modification to account for a reduced sample size, total CK activity of the capillary and venous samples was analyzed concurrently via spectrophotometry. Results indicated a 0.997 correlation between sampling sites for total CK, with disagreement between the venous and capillary samples estimated at <12% across the range of CK values. These findings indicate capillary sampling for total CK activity provides a valid alternative to venipuncture and should be considered by researchers, clinicians, and strength and conditioning specialists as an alternate sampling technique when indirectly evaluating muscle damage after exercise.
Authors:
Mark A Knoblauch; Daniel P O'Connor; Mark S F Clarke
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of strength and conditioning research / National Strength & Conditioning Association     Volume:  24     ISSN:  1533-4287     ISO Abbreviation:  J Strength Cond Res     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9415084     Medline TA:  J Strength Cond Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3471-5     Citation Subset:  IM    
Affiliation:
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, Texas, USA. maknoblauch@gmail.com
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