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Capillary liquid chromatography separations using non-porous pillar array columns.
MedLine Citation:
PMID:  22341659     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We report on a series of explorative experiments wherein a non-porous pillar array column (NP-PAC) is coupled to a commercial capillary LC instrument. The performance of the system was evaluated by both non-retained and retained experiments using several types of samples. In order to minimize interfacing related dispersion, relatively large pillars (d(p)=11μm) were defined so that a considerable depth could be achieved (50μm), resulting in an equivalent cylindrical internal diameter of 252μm. Connecting 20 channel tracks of 1mm wide and 7cm long by previously developed distributor-based turns, a large channel length (1.4m) and volume (28μl) could be achieved without compromising the separation performance excessively. Establishing a van Deemter curve under non-retained conditions with off-chip injection and detection, a minimal plate height of 13μm was established, resulting in some 100,000 plates obtained in 30min. To demonstrate the practical applicability of the NP-PAC, high pressure operation was applied to perform a number of example separations (parabens, phenones, sulfonamides, steroids and BSA digest) during a continuous operation period of 3 months wherein some 500 injections were performed. In the gradient mode, the NP-PAC approach allowed to achieve good to reasonable peak capacities (n(p)=100-140 in 50-70min) and symmetries for both large and small solutes and for both gradient and isocratic separation modes, with figures of merit for the quantification of the peaks in the ppm range, opening more perspectives for microfluidics-based small molecule analysis.
Authors:
Wim De Malsche; Selm De Bruyne; Jeff Op De Beek; Pat Sandra; Han Gardeniers; Gert Desmet; Frederic Lynen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-25
Journal Detail:
Title:  Journal of chromatography. A     Volume:  -     ISSN:  1873-3778     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-2-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
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