| Capillary gas chromatographic separation of bile acid acyl glycosides without thermal decomposition and isomerization. | |
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MedLine Citation:
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PMID: 15584236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A direct method for the capillary gas chromatographic (cGC) separation of the acyl glycosides of bile acids was successfully attained. The free acyl glycosides were derivatized to their complete trifluoroacetyl (TFA) derivatives with N-methyl-bis(trifluoroacetamide). The highly volatile TFA derivatives were chromatographed on a short-length (10 m), narrow-bore (0.1 mm) capillary column coated with a thin film (0.1 microm) of 5% phenyl polysilphenylene-siloxane at a column temperature below 280 degrees C. Each exhibited a single, well-separated peak of the theoretical shape without any accompanying peaks due to the thermal decomposition and isomerization. The bile acid 24alpha-glucosides were always eluted faster than the corresponding 24beta-glucosides, which eluted before the corresponding 24beta-galactosides. The method could be usefully applied to biosynthetic and metabolic studies of bile acid acyl glycosides in biological materials. |
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Authors:
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Takashi Iida; Shoujiro Ogawa; Genta Kakiyama; Takaaki Goto; Nariyasu Mano; Junichi Goto; Toshio Nambara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1057 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-12-08 Completed Date: 2005-04-18 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 171-6 Citation Subset: IM |
Affiliation:
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Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajousui, Setagaya, Tokyo 156-8550, Japan. takaiida@chs.nihon-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bile Acids and Salts
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isolation & purification* Chromatography, Gas / methods* Glycosides / isolation & purification* Hot Temperature Isomerism Spectrometry, Mass, Electrospray Ionization |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Glycosides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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