Document Detail


Capillary electrophoretic analysis of brimonidine in aqueous humor of the eye and blood sera and relation of its levels with intraocular pressure.
MedLine Citation:
PMID:  10960828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was the development of a capillary electrophoretic method for the determination of the levels of the selective alpha(2)-adrenergic receptor agonist brimonidine in aqueous humor of the eye and blood sera and their relation to its efficacy in reducing the intraocular pressure (IOP). Analysis of brimonidine was performed by capillary zone electrophoresis using 20 mM borate, pH 9.3, as operating buffer and detection at 255 nm. Brimonidine levels were determined in aqueous humor and blood sera from seven patients admitted for cataract extraction following ocular administration of the ophthalmic Alphagantrade mark solution. Levels of brimonidine and IOP values were recorded for a 24 h period. Alphagantrade mark administration resulted in a significant reduction of IOP, from within 30 min up to 4-5 h, whereafter a stepwise increase was recorded until 24 h, where mean IOP value returned to that before administration. The IOP reduction was related to the levels of brimonidine in aqueous humor, where maximal levels (80-100%) were obtained within 1-3 h. A 50% amount of the solution was determined after 4-5 h, whereas it reached the minimum level after 12 h. Serum levels reached maximum within 3-4 h, a 50% reduction was recorded in 12 h and minimum level in 24 h. It is concluded that brimonidine administration may significantly reduce IOP in patients when its level is maintained >/=50% of the maximum present in aqueous humor, i.e within a 4-6 h period. Since at this time the level of brimonidine in blood serum has reached maximum value, administration of brimonidine every 6 h may be used to obtain adequate brimonidine levels to maintain a constantly lowered IOP.
Authors:
D N Tzovolou; F Lamari; E K Mela; S P Gartaganis; N K Karamanos
Related Documents :
7549448 - Systemic blood pressure and capillary blood-cell velocity in glaucoma patients: a preli...
10206218 - Evaluation of ocular arterial changes in glaucoma with color doppler ultrasonography.
305728 - The dose-response relationships of dipivalyl epinephrine in open-angle glaucoma.
17106408 - A clinical evaluation of proview pressure phosphene tonometry in children.
8969878 - Pressure on acupoints decreases postoperative pain.
6762038 - Techniques of ventilatory therapy in the adult respiratory distress syndrome (ards).
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  14     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-25     Completed Date:  2000-09-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  301-5     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Affiliation:
Section of Organic Chemistry, Biochemistry and Natural Products, Department of Chemistry, University of Patras, 261 10 Patras, Greece.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adrenergic alpha-Agonists / analysis*,  blood,  pharmacology
Aged
Aqueous Humor / chemistry*
Calibration
Electrophoresis, Capillary / methods*
Female
Humans
Intraocular Pressure / drug effects*
Male
Ophthalmic Solutions / chemistry
Quinoxalines / analysis*,  blood,  pharmacology
Chemical
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Ophthalmic Solutions; 0/Quinoxalines; 59803-98-4/brimonidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluore...
Next Document:  Quantitative determination of melatonin in human plasma and cerebrospinal fluid with high-performanc...