Document Detail


Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: food and biological fluids.
MedLine Citation:
PMID:  23386346     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Sample preparation and laser-induced fluorescence detection are two key steps of the analytical methodology to determine by capillary electrophoresis low concentrations of proteins in complex sample matrices. In this chapter the options of performing both steps in different ways are shown by detailing the analysis of the allergen β-lactoglobulin in food products for infants and the analysis of the isoforms of alpha 1-acid glycoprotein, a potential biomarker, in serum and secretome.
Authors:
Raul Garrido-Medina; Angel Puerta; Cristina Pelaez-Lorenzo; Zuly Rivera-Monroy; Andras Guttman; Jose Carlos Diez-Masa; Mercedes de Frutos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  984     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-02-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  207-25     Citation Subset:  IM    
Affiliation:
Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain.
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