Document Detail

Capillary electrophoresis of single mammalian cells.
MedLine Citation:
PMID:  15174037     Owner:  NLM     Status:  MEDLINE    
Capillary electrophoresis (CE) has been established as powerful tool for single cell analysis. Newly developed sampling, separation and detection methods have allowed the investigation of single mammalian cells with CE despite their small size and complex composition. Advances in sample injection techniques include several novel methods for the injection of whole cells and sampling techniques for the study of cellular secretion. CE of single mammalian cells has been applied in a wide range of fields including protein analysis, neuroscience, and oncology. The development of new detection schemes in the analysis of single mammalian cells with CE has included studies of protein expression and the utilization of mass spectrometric and electrochemical detection. Subcellular mammalian cell analysis with CE also has been investigated.
Lori A Woods; Thomas P Roddy; Andrew G Ewing
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Electrophoresis     Volume:  25     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-06-02     Completed Date:  2005-01-24     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1181-7     Citation Subset:  IM    
Department of Chemistry, The Pennsylvania State University, University Park, PA, USA.
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MeSH Terms
Cells / metabolism*,  secretion
Electrophoresis, Capillary
Proteins / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Reg. No./Substance:

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