Document Detail

Capillary electrophoresis for monitoring dityrosine and 3-bromotyrosine synthesis.
MedLine Citation:
PMID:  16384575     Owner:  NLM     Status:  MEDLINE    
Protein oxidation affects the structure of many amino acids. Variants of tyrosine are increasingly important in medical and food sciences. The synthesis of standards is essential for monitoring the disease state of patients with various illnesses and the quality of a number of food products. A method for monitoring standard synthesis of dityrosine and 3-bromotyrsoine from tyrosine using capillary electrophoresis (CE) is presented. Optimum separation was achieved using an isoelectric buffer consisting of 100mM iminodiacetic acid (IDA)+75 mM lauryl sulfobetaine (SB 3-12)+0.02% hydroxypropyl methylcellulose (HPMC) in a 27 cm x 75 microm capillary at 22 kV and 45 degrees C. Using these conditions the tyrosine adducts could be easily separated in less than 4 min. The resolution of the CE method was similar to HPLC separations, but analysis time was distinctly shorter.
Michael Tilley; Scott R Bean; Katherine A Tilley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2005-12-27
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1103     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-03-10     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  368-71     Citation Subset:  IM    
US Department of Agriculture, Agricultural Research Service Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA.
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MeSH Terms
Electrophoresis, Capillary / methods*
Tyrosine / analogs & derivatives*,  chemical synthesis
Reg. No./Substance:
54788-30-6/3-bromotyrosine; 55520-40-6/Tyrosine; 980-21-2/dityrosine

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