Document Detail


Capillary electrophoresis for monitoring dityrosine and 3-bromotyrosine synthesis.
MedLine Citation:
PMID:  16384575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein oxidation affects the structure of many amino acids. Variants of tyrosine are increasingly important in medical and food sciences. The synthesis of standards is essential for monitoring the disease state of patients with various illnesses and the quality of a number of food products. A method for monitoring standard synthesis of dityrosine and 3-bromotyrsoine from tyrosine using capillary electrophoresis (CE) is presented. Optimum separation was achieved using an isoelectric buffer consisting of 100mM iminodiacetic acid (IDA)+75 mM lauryl sulfobetaine (SB 3-12)+0.02% hydroxypropyl methylcellulose (HPMC) in a 27 cm x 75 microm capillary at 22 kV and 45 degrees C. Using these conditions the tyrosine adducts could be easily separated in less than 4 min. The resolution of the CE method was similar to HPLC separations, but analysis time was distinctly shorter.
Authors:
Michael Tilley; Scott R Bean; Katherine A Tilley
Related Documents :
21356695 - Assessment of the olfactory response to chemicals or bacteria in pristionchus nematodes.
21221965 - Carbaryl concentration gradients in realistic environments and their influence on our u...
10552395 - Optimization of the synthesis of the cross-linked amino acid ornithinoalanine and nucle...
3456795 - Overjet and overbite distribution and correlation: a comparative epidemiological englis...
18986595 - Food balance sheet and household budget survey dietary data and mortality patterns in e...
15820615 - Unsaturated fatty acids intake and all-causes mortality: a 8.5-year follow-up of the it...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2005-12-27
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1103     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-03-10     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  368-71     Citation Subset:  IM    
Affiliation:
US Department of Agriculture, Agricultural Research Service Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA. Michael.tilley@gmprc.ksu.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Capillary / methods*
Tyrosine / analogs & derivatives*,  chemical synthesis
Chemical
Reg. No./Substance:
54788-30-6/3-bromotyrosine; 55520-40-6/Tyrosine; 980-21-2/dityrosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Predicting the behaviour of proteins in hydrophobic interaction chromatography. 2. Using a statistic...
Next Document:  Polarity parameters of the Symmetry C18 and Chromolith Performance RP-18 monolithic chromatographic ...