Document Detail


Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood.
MedLine Citation:
PMID:  18723405     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasma aromatic and sulfur containing amino acids are good indicators of protein anabolism/catabolism, while blood reduced and oxidized glutathione reflect oxidative status in an organism. Using a full factorial design for screening important variables (pH, concentration, temperature) we developed a capillary zone electrophoresis method permitting their measurements in the single run, without any derivatization procedures. The best separations were obtained within less than 30 min employing a 10 mmol/l phosphate buffer, pH 2.8, 18 degrees C, 15 kV voltage. Fairly good precision with a linear relationship between peak area and concentrations (r=0.995-0.999) were obtained. The method was used to analyze human capillary blood.
Authors:
Gordana Zunić; Slavica Spasić
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-03
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  873     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-09     Completed Date:  2008-11-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  70-6     Citation Subset:  IM    
Affiliation:
Institute for Medical Research, Military Medical Academy, Belgrade, Serbia. slavica@pharmacy.bg.ac.yu
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / blood*,  isolation & purification
Capillaries
Electrophoresis, Capillary
Glutathione / blood*,  isolation & purification
Glutathione Disulfide / blood
Humans
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Amino Acids; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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