Document Detail

Capillary electrophoresis of heparin and dermatan sulfate unsaturated disaccharides with triethylamine and acetonitrile as electrolyte additives.
MedLine Citation:
PMID:  8767748     Owner:  NLM     Status:  MEDLINE    
Capillary electrophoresis at constant voltage with the addition of triethylamine as electrolyte to a running buffer containing borate using fused-silica capillaries permits the complete resolution in less than 30 min of 11 standard heparin and 8 standard dermatan sulfate disaccharides, which represent degradation products of heparin and dermatan sulfate by specific lyases. Triethylamine influences the migration time of disaccharides by reducing both their electrophoretic mobility towards the anode and the electroosmotic flow towards the cathode. A modulated combination of these effects together with borate-disaccharide complex formation is responsible for separation, especially in the case of isomers which differ in the position of the sulfate groups. The addition of acetonitrile did not introduce any favourable effect in the separation of disaccharide mixtures. Under these conditions different dermatan sulfates were analysed to assess the source of the preparations.
L Scapol; E Marchi; G C Viscomi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  735     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-09-16     Completed Date:  1996-09-16     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  367-74     Citation Subset:  IM    
Alfa Wassermann S.p.A., Bologna, Italy.
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MeSH Terms
Boric Acids
Carbohydrate Sequence
Dermatan Sulfate / isolation & purification*
Disaccharides / isolation & purification*
Electrophoresis, Capillary / methods*
Heparin / isolation & purification*
Macromolecular Substances
Molecular Sequence Data
Skin / chemistry
Reg. No./Substance:
0/Acetonitriles; 0/Boric Acids; 0/Buffers; 0/Disaccharides; 0/Ethylamines; 0/Macromolecular Substances; 11113-50-1/boric acid; 121-44-8/triethylamine; 24967-94-0/Dermatan Sulfate; 75-05-8/acetonitrile; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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