Document Detail


Capillary electrophoresis of free amino acids in physiological fluids without derivatization employing direct or indirect absorbance detection.
MedLine Citation:
PMID:  22125149     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Whole blood and/or plasma amino acids are useful for monitoring whole body protein and amino acid metabolism in an organism under various physiological and pathophysiological conditions. Various methodological procedures are in use for their measurement in biological fluids. From the time when capillary electrophoresis was introduced as a technology offering rapid separation of various ionic and/or ionizable compounds with low sample and solvent consumption, there were many attempts to use it for the measurement of amino acids present in physiological fluids. As a rule, these methods require derivatization procedures for detection purposes.Here, we present two protocols for the analysis of free amino acids employing free zone capillary electrophoresis. Main advantage of both methods is an absence of any derivatization procedures that permits the analysis of free amino acid in physiological fluids. The method using direct detection and carrier electrolyte consisting of disodium monophosphate (10 mM at pH 2.90) permits determination of compounds that absorb in UV region (aromatic and sulfur containing amino acids, as well as some peptides, such as carnosine, reduced and oxidized glutathione). The other method uses indirect absorbance detection, employing 8 mM p-amino salicylic acid buffered with sodium carbonate at pH 10.2 as running electrolyte. It permits quantification of 30 underivatized physiological amino acids and peptides. In our experience, factorial design represents a useful tool for final optimization of the electrophoretic conditions if it is necessary.
Authors:
Gordana D Zunić; Slavica Spasić; Zorana Jelić-Ivanović
Related Documents :
11152589 - Spectrophotometric measurement of mercaptans with 4,4'-dithiodipyridine.
3155519 - Formation of a beta-aspartyl phosphate intermediate by the vanadate-sensitive atpase of...
18272199 - Synthesis and bioorthogonal coupling chemistry of a novel cyclopentenone-containing unn...
8902259 - The reaction of 2-(methylseleno)benzanilide with peroxynitrite.
2900739 - Metabolism of 1-chloro-2-methylpropene. evidence for reactive chloroaldehyde intermedia...
17386529 - Dimethylthioarsinic anhydride: a standard for arsenic speciation.
2540689 - Evaluation of the causal relationship between crocidolite asbestos-induced lipid peroxi...
3917929 - Schistosoma mansoni: eicosanoid production by cercariae.
1424159 - Identification of urinary metabolites of (+/-)-2-(p-isobutylphenyl)propionic acid (ibup...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  828     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-54     Citation Subset:  IM    
Affiliation:
Institute for Medical Research, Military Medical Academy, Belgrade, Serbia, gordana144@open.telekom.rs.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Quantification of Underivatised Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC-ESI-MS/MS.
Next Document:  Measurement of 3-nitro-tyrosine in human plasma and urine by gas chromatography-tandem mass spectrom...