Document Detail


Capillary electrophoresis of chitooligosaccharides in acidic solution: simple determination using a quaternary-ammonium-modified column and indirect photometric detection with crystal violet.
MedLine Citation:
PMID:  20004627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Five chitosan oligosaccharides were separated in acidic aqueous solution by capillary electrophoresis (CE) with indirect photometric detection using a positively coated capillary. Electrophoretic mobility of the chitooligosaccharides (COSs) depended on the number of monomer units in acidic aqueous solution, similar to other polyelectrolyte oligomers. The separation was developed in nitric acid aqueous solution at pH 3.0 with 1 mM Crystal Violet, using a capillary positively coated with N-trimethoxypropyl-N,N,N-trimethylammonium chloride. The limit of the detection for chitooligosaccharides with two to six saccharide chains was less than 5 microM. CE determination of an enzymatically hydrolyzed COS agreed with results from HPLC.
Authors:
Toshiaki Hattori; Nobuhiro Anraku; Ryo Kato
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Publication Detail:
Type:  Journal Article     Date:  2009-12-01
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  878     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-04     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  477-80     Citation Subset:  IM    
Copyright Information:
2009 Elsevier B.V. All rights reserved.
Affiliation:
Department of Materials Science, Toyohashi University of Technology, Hibarigaoka 1-1, Tempaku, Toyohashi 441-8580, Japan. thattori@tutms.tut.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Chitosan / analysis*,  chemistry
Electrophoresis, Capillary / methods*
Gentian Violet / chemistry*
Hydrolysis
Motion
Nitric Acid / chemistry*
Oligosaccharides / analysis*,  chemistry
Onium Compounds / chemistry
Organophosphorus Compounds / chemistry
Photochemistry / instrumentation*
Quaternary Ammonium Compounds / chemistry*
Reference Standards
Solutions
Chemical
Reg. No./Substance:
0/Oligosaccharides; 0/Onium Compounds; 0/Organophosphorus Compounds; 0/Quaternary Ammonium Compounds; 0/Solutions; 18198-39-5/tetraphenylphosphonium; 548-62-9/Gentian Violet; 7697-37-2/Nitric Acid; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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