Document Detail

Capillary electrophoresis of arsenic compounds with indirect fluorescence detection.
MedLine Citation:
PMID:  9761195     Owner:  NLM     Status:  MEDLINE    
A capillary electrophoresis (CE)-indirect fluorescence detection method for arsenic compounds is described. The five arsenic species, viz., arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), dimethylarsinate (DMA) and phenylarsonate (PhA), were efficiently separated by CE in 8 min with an 1.5 mM fluorescein solution at pH 9.8. Fluorescein also functioned as a background fluorophore for the indirect detection of these nonfluorescent arsenic species. Linearity (r> or =0.996) of more than two orders of magnitude was generally obtained. The relative standard deviation (RSD) values were in the ranges 0.4-0.7% and 2.2-8.2% for migration times and peak areas, respectively. The concentration limits of detection (CLODs) for the arsenic compounds studied were between 0.04 and 0.16 microg/mL (as arsenic). The detection sensitivity was generally dependent upon the transfer ratio (TR, defined as the number of moles of fluorescein ions displaced by one mole of analyte ions) of each arsenic species. The applicability of the method for the analysis of ground water was examined.
Y M Huang; C W Whang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  19     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-11     Completed Date:  1998-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  2140-4     Citation Subset:  IM    
Department of Chemistry, Tunghai University, Taichung, Taiwan.
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MeSH Terms
Arsenicals / isolation & purification*
Electrophoresis, Capillary / methods*
Hydrogen-Ion Concentration
Quality Control
Sensitivity and Specificity
Reg. No./Substance:
0/Arsenicals; 0/Buffers; 2321-07-5/Fluorescein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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