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Capillary electrophoresis analysis of organic amines and amino acids in saline and acidic samples using the Mars organic analyzer.
MedLine Citation:
PMID:  19968460     Owner:  NLM     Status:  In-Process    
The Mars Organic Analyzer (MOA) has enabled the sensitive detection of amino acid and amine biomarkers in laboratory standards and in a variety of field sample tests. However, the MOA is challenged when samples are extremely acidic and saline or contain polyvalent cations. Here, we have optimized the MOA analysis, sample labeling, and sample dilution buffers to handle such challenging samples more robustly. Higher ionic strength buffer systems with pK(a) values near pH 9 were developed to provide better buffering capacity and salt tolerance. The addition of ethylaminediaminetetraacetic acid (EDTA) ameliorates the negative effects of multivalent cations. The optimized protocol utilizes a 75 mM borate buffer (pH 9.5) for Pacific Blue labeling of amines and amino acids. After labeling, 50 mM (final concentration) EDTA is added to samples containing divalent cations to ameliorate their effects. This optimized protocol was used to successfully analyze amino acids in a saturated brine sample from Saline Valley, California, and a subcritical water extract of a highly acidic sample from the Río Tinto, Spain. This work expands the analytical capabilities of the MOA and increases its sensitivity and robustness for samples from extraterrestrial environments that may exhibit pH and salt extremes as well as metal ions.
Amanda M Stockton; Thomas N Chiesl; Tim K Lowenstein; Xenia Amashukeli; Frank Grunthaner; Richard A Mathies
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Astrobiology     Volume:  9     ISSN:  1557-8070     ISO Abbreviation:  Astrobiology     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-12-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088083     Medline TA:  Astrobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  823-31     Citation Subset:  IM    
Department of Chemistry, University of California, Berkeley, California 94720, USA.
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