Document Detail


Capillary Electrophoresis of Free Fatty Acids by Indirect UV Detection: Application to the Classification of Vegetable Oils According to Their Botanical Origin.
MedLine Citation:
PMID:  21905655     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A method for the determination of fatty acids in vegetable oils by capillary electrophoresis with indirect UV-Vis detection has been developed. The separation of fatty acids was optimized in terms of Brij surfactant nature and concentration, and organic modifier (2-propanol) percentage. The optimal background electrolyte consisted of a 10 mM p-hydroxybenzoate, 5 mM Tris at pH 8.8, 80 mM Brij 98, 40% acetonitrile and 10% 2-propanol. Under these conditions, vegetable oils from five botanical origins (avocado, corn, extra virgin olive, hazelnut and soybean) were analyzed and the fatty acid content established. Linear discriminant analysis (LDA) models were constructed using fatty acid peak areas (selected by pairs) as predictors. An excellent resolution among all category pairs was obtained, being all samples correctly classified with assignment probabilities higher than 95%.
Authors:
María Vergara-Barberán; Aarón Escrig-Doménech; María Jesús Lerma-Garcia; Ernesto Francisco Simo-Alfonso; Jose Manuel Herrero-Martínez
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-9
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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